The expression of caffeic acid 3-O-methyltransferase in two wheat genotypes differing in lodging resistance.
The expression of caffeic acid 3-O-methyltransferase in two wheat genotypes differing in lodging resistance.
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DOI：
10.1093/jxb/erp132
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发表时间：
2009
影响因子：
6.9
通讯作者：
Ma QH
中科院分区：
文献类型：
--
作者：
Ma QH
茎秆抗倒伏是作物生产中的一个重要表型。以Zadoks期Z37、Z60和Z75对应的抗倒伏（H4564）和易倒伏（C6001）品种茎秆伸长、抽穗和乳状胚乳的基部第二节间为材料，测定了COMT基因（TaCM）的表达。用蛋白凝胶印迹法分析了不同茎发育阶段的TaCM蛋白水平，并测定了COMT酶活性。H4546在拔节至乳熟期TaCM mRNA水平较高，而C6001在抽穗期和乳熟期TaCM mRNA水平显著降低。抽穗期和乳浊期，H4564的TaCM蛋白水平和COMT活性均高于C6001。这些结果表明，抽穗期和乳白期，H4564的木质素含量和茎强均高于C6001，倒伏指数低于C6001。因此，两个小麦品种茎秆发育过程中TaCM mRNA水平、蛋白水平和酶活性与茎秆强度和倒伏指数相关。对不同群体的Southern分析表明，TaCM位点位于3BL染色体远端区域，该区域在抗倒立表型上的QTL研究较少。
Stem lodging-resistance is an important phenotype in crop production. In the present study, the expression of the wheat COMT gene (TaCM) was determined in basal second internodes of lodging-resistant (H4564) and lodging-susceptible (C6001) cultivars at stem elongation, heading, and milky endosperm corresponding to Zadoks stages Z37, Z60, and Z75, respectively. The TaCM protein levels were analysed by protein gel blot and COMT enzyme activity was determined during the same stem developmental stages. TaCM mRNA levels were higher in H4546 from elongation to the milky stages and in C6001 the TaCM mRNA levels decreased markedly at the heading and milky stages. The TaCM protein levels and COMT activity were also higher in H4564 than that in C6001 at the heading and milky stages. These results corresponded to a higher lignin content measured by the Klason method and stem strength and a lower lodging index in H4564 than in C6001 at the heading and milky stages. Therefore, the TaCM mRNA levels, protein levels, and enzyme activity in developing wheat stems were associated with stem strength and lodging index in these two wheat cultivars. Southern analysis in a different population suggested that a TaCM locus was located in the distal region of chromosome 3BL, which has less investigated by QTL for lodging-resistant phenotype.
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影响因子：
5.1
作者：
COLLAZO, P;MONTOLIU, L;RIGAU, J
通讯作者：
RIGAU, J
影响因子：
3.7
作者：
Ma, QH;Tian, B
通讯作者：
Tian, B
影响因子：
5.4
作者：
Huang, X. Q.;Cloutier, S.;Brown, P. D.
通讯作者：
Brown, P. D.
影响因子：
4.3
作者：
Ishimaru, Ken;Togawa, Eiji;Hirotsu, Naoki
通讯作者：
Hirotsu, Naoki
影响因子：
2.9
作者：
Kersey, R;Inoue, K;Dixon, RA
通讯作者：
Dixon, RA