Variations in the NSP4 gene of the type 2 porcine reproductive and respiratory syndrome virus isolated in China from 1996 to 2021.
Variations in the NSP4 gene of the type 2 porcine reproductive and respiratory syndrome virus isolated in China from 1996 to 2021.
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DOI：
10.1007/s11262-022-01957-x
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发表时间：
2023-02
期刊：
影响因子：
1.6
通讯作者：
中科院分区：
文献类型：
--
作者：
猪繁殖与呼吸综合征病毒（PRRSV）自1996年在我国首次分离以来不断变异，导致感染防控困难。系统发育分析表明，PRRSV-2毒株引起的感染是中国的主要流行毒株。在这项研究中，我们重点研究了过去 20 年中国 PRRSV-2 非结构蛋白 4 (NSP4) 的流行情况和遗传变异。预测了NSP4的基本生物学特性，并利用123株PRRSV-2菌株对NSP4在核苷酸和氨基酸水平上的同源性进行了分析和比较。 NSP4蛋白的预测分子量确定为21.1 kDa，并且预测它是一种缺乏信号肽的稳定的疏水性蛋白。不同菌株的NSP4表现出高度的氨基酸（85.8-100%）和核苷酸序列同源性（81.0-100%）。在 15 种代表性 PRRSV-2 毒株的 NSP4 中发现了多个氨基酸取代。系统发育分析表明，8系和1系是中国最流行的菌株，是遗传距离较远的无差异支系。该分析将有助于全面阐明中国PRRSV NSP4流行的参数，为充分了解NSP4的功能奠定基础。遗传信息来自保守和非保守序列的积累。 NSP4基因的高度保守性决定了PRRSV最基本的生命性状和功能。分析NSP4蛋白的空间结构，研究NSP4的遗传进化，不仅为NSP4如何参与调节宿主先天反应提供理论依据，而且为基因操作提供靶点，为新药物分子提供合理的靶分子和结构。在线版本包含可在 10.1007/s11262-022-01957-x 获取的补充材料。
Porcine reproductive and respiratory syndrome virus (PRRSV) has continuously mutated since its first isolation in China in 1996, leading to difficulties in infection prevention and control. Infections caused by PRRSV-2 strains are the main epidemic strains in China, as determined by phylogenetic analysis. In this study, we focused on the prevalence and genetic variations of the non-structural protein 4 (NSP4) from PRRSV-2 over the past 20 years in China. The fundamental biological properties of the NSP4 were predicted, and an analysis and comparison of NSP4 homology at the nucleotide and amino acid levels was conducted using 123 PRRSV-2 strains. The predicted molecular weight of the NSP4 protein was determined to be 21.1 kDa, and it was predicted to be a stable hydrophobic protein that lacks a signal peptide. NSP4 from different strains exhibited a high degree of amino acid (85.8–100%) and nucleotide sequence homology (81.0–100%). Multiple amino acid substitutions were identified in NSP4 among 15 representative PRRSV-2 strains. Phylogenetic analysis showed that the lineage 8 and 1 strains, the most prevalent strains in China, were indifferent clades with a long genetic distance. This analysis will help fully elucidate the parameters of the PRRSV NSP4 epidemic in China to lay a foundation for adequate understanding of the function of NSP4. Genetic information results from the accumulation of conserved and non-conserved sequences. The high conservation of the NSP4 gene determines the most basic life traits and functions of PRRSV. Analyzing the spatial structure of NSP4 protein and studying the genetic evolution of NSP4 not only provide the theoretical basis for how NSP4 participates in the regulation of the innate response of the host but also provide a target for genetic manipulation and a reasonable target molecule and structure for new drug molecules. The online version contains supplementary material available at 10.1007/s11262-022-01957-x.
影响因子：
2.7
作者：
Xu AT;Zhou YJ;Li GX;Yu H;Yan LP;Tong GZ
通讯作者：
Tong GZ