Aberrant lipid accumulation in the mouse visceral yolk sac resulting from maternal diabetes and obesity.
Aberrant lipid accumulation in the mouse visceral yolk sac resulting from maternal diabetes and obesity.
复制标题
母体糖尿病和肥胖引起的小鼠内脏蛋黄囊中异常脂质积累。
DOI：
10.3389/fcell.2023.1073807
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发表时间：
2023
影响因子：
5.5
通讯作者：
Kappen, Claudia
中科院分区：
文献类型：
--
作者：
Zhang, Man;Salbaum, J. Michael;Jones, Sydney;Burk, David;Kappen, Claudia
关键词：
母亲怀孕期间的糖尿病和肥胖是众所周知的结构性出生缺陷的危险因素，包括神经管缺陷和先天性心脏缺陷。受影响妊娠的后代也容易在以后的生活中患上心脏代谢性疾病。基于大鼠胚胎的体外胚胎培养，推测糖尿病妊娠时卵黄囊对营养物质的摄取不足。相比之下，使用两个独立的母体糖尿病小鼠模型和一个母体肥胖症的高脂饮食喂养模型，我们观察到卵黄囊在8.5天时脂肪过度积累。糖尿病和肥胖者胚胎卵黄囊内脂滴的数量和大小均增加。母体代谢性疾病不影响脂转运蛋白的表达，包括ApoA1、ApoB和SR-B1，这与我们早先报道的糖尿病妊娠卵黄囊中葡萄糖和脂肪酸转运蛋白基因的表达也没有变化一致。与正常妊娠的卵黄囊相比，糖尿病和肥胖症患者卵黄囊中脂滴与溶酶体的共存显著减少。因此，我们得出结论，在受母体代谢性疾病影响的妊娠中，脂质的处理是有缺陷的，这可能会导致发育中的胚胎获得的脂质减少。文中还讨论了脂质供应不足--以及可能的其他营养物质--对经历不利妊娠条件的胚胎的可能影响。
Maternal diabetes and obesity in pregnancy are well-known risk factors for structural birth defects, including neural tube defects and congenital heart defects. Progeny from affected pregnancies are also predisposed to developing cardiometabolic disease in later life. Based upon in vitro embryo cultures of rat embryos, it was postulated that nutrient uptake by the yolk sac is deficient in diabetic pregnancies. In contrast, using two independent mouse models of maternal diabetes, and a high-fat diet-feeding model of maternal obesity, we observed excessive lipid accumulation at 8.5 days in the yolk sac. The numbers as well as sizes of intracellular lipid droplets were increased in yolk sacs of embryos from diabetic and obese pregnancies. Maternal metabolic disease did not affect expression of lipid transporter proteins, including ApoA1, ApoB and SR-B1, consistent with our earlier report that expression of glucose and fatty acid transporter genes was also unchanged in diabetic pregnancy-derived yolk sacs. Colocalization of lipid droplets with lysosomes was significantly reduced in the yolk sacs from diabetic and obese pregnancies compared to yolk sacs from normal pregnancies. We therefore conclude that processing of lipids is defective in pregnancies affected by maternal metabolic disease, which may lead to reduced availability of lipids to the developing embryo. The possible implications of insufficient supply of lipids -and potentially of other nutrients-to the embryos experiencing adverse pregnancy conditions are discussed.
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影响因子：
3.5
作者：
Martínez-Frías, ML;Frías, JP;Frías, JL
通讯作者：
Frías, JL
影响因子：
8.2
作者：
BAKER, L;PIDDINGTON, R;MOEHRING, J
通讯作者：
MOEHRING, J
DOI：
10.1002/tera.1420450213
发表时间：
1992-02-01
期刊：
TERATOLOGY
影响因子：
--
作者：
HUNTER, ES;SADLER, TW
通讯作者：
SADLER, TW
影响因子：
5.5
作者：
通讯作者：
--
影响因子：
15.9
作者：
HUANG, LS;VOYIAZIAKIS, E;BRESLOW, JL
通讯作者：
BRESLOW, JL