Nutrient Transporter Gene Expression in the Early Conceptus-Implications From Two Mouse Models of Diabetic Pregnancy.
Nutrient Transporter Gene Expression in the Early Conceptus-Implications From Two Mouse Models of Diabetic Pregnancy.
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DOI：
10.3389/fcell.2022.777844
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发表时间：
2022
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
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妊娠早期母亲患有糖尿病会增加后代出生缺陷的风险，特别是心脏和神经管缺陷。虽然血糖水平升高是糖尿病妊娠的特征，但这些也伴随着高脂血症，表明营养可用性发生改变。因此，我们研究了受孕部位或植入后早期胚胎中营养转运蛋白表达的变化是否可以解释发育后期出生缺陷发生率的增加。我们重点关注葡萄糖和脂肪酸转运蛋白，通过 RT-PCR 测量了它们在自发性糖尿病非肥胖小鼠品系 NOD 和患有链脲佐菌素诱导糖尿病的妊娠 FVB/N 小鼠品系母鼠中的表达。通过RNAscope原位杂交确定蜕膜、胚胎外和胚胎组织中的表达位点。虽然母亲糖尿病对表达水平或细胞谱没有明显影响，但我们检测到特定营养转运蛋白的惊人细胞类型特异性。例如，Slc2a2/Glut2表达仅限于内脏卵黄囊的内胚层细胞，而Slc2a1/Glut1表达仅限于中胚层隔室； Slc27a4/Fatp4 和 Slc27a3/Fatp3 也分别在卵黄囊的内胚层和中胚层区室中表现出相互排斥的表达。这些发现不仅强调了营养转运蛋白在子宫内环境中的重要性，而且对糖尿病妊娠出生缺陷的病因学以及旨在通过营养补充降低出生缺陷风险的策略提出了重要意义。
Maternal diabetes in early pregnancy increases the risk for birth defects in the offspring, particularly heart, and neural tube defects. While elevated glucose levels are characteristic for diabetic pregnancies, these are also accompanied by hyperlipidemia, indicating altered nutrient availability. We therefore investigated whether changes in the expression of nutrient transporters at the conception site or in the early post-implantation embryo could account for increased birth defect incidence at later developmental stages. Focusing on glucose and fatty acid transporters, we measured their expression by RT-PCR in the spontaneously diabetic non-obese mouse strain NOD, and in pregnant FVB/N mouse strain dams with Streptozotocin-induced diabetes. Sites of expression in the deciduum, extra-embryonic, and embryonic tissues were determined by RNAscope in situ hybridization. While maternal diabetes had no apparent effects on levels or cellular profiles of expression, we detected striking cell-type specificity of particular nutrient transporters. For examples, Slc2a2/Glut2 expression was restricted to the endodermal cells of the visceral yolk sac, while Slc2a1/Glut1 expression was limited to the mesodermal compartment; Slc27a4/Fatp4 and Slc27a3/Fatp3 also exhibited reciprocally exclusive expression in the endodermal and mesodermal compartments of the yolk sac, respectively. These findings not only highlight the significance of nutrient transporters in the intrauterine environment, but also raise important implications for the etiology of birth defects in diabetic pregnancies, and for strategies aimed at reducing birth defects risk by nutrient supplementation.
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影响因子：
1.3
作者：
Banhidy, Ferenc;Dakhlaoui, Abdallah;Czeizel, Andrew A. E.
通讯作者：
Czeizel, Andrew A. E.
影响因子：
4.8
作者：
Gimeno, RE;Hirsch, DJ;Stahl, A
通讯作者：
Stahl, A
影响因子：
5.8
作者：
Basu, Arpita;Alaupovic, Petar;Lyons, Timothy J.
通讯作者：
Lyons, Timothy J.
DOI：
10.1159/000242138
发表时间：
1985-01-01
期刊：
BIOLOGY OF THE NEONATE
影响因子：
--
作者：
GOLDSTEIN, R;LEVY, E;SHAFRIR, E
通讯作者：
SHAFRIR, E
DOI：
10.1002/tera.1420160205
发表时间：
1977-01-01
期刊：
TERATOLOGY
影响因子：
--
作者：
COCKROFT, DL;COPPOLA, PT
通讯作者：
COPPOLA, PT