Feeding a high-concentrate corn straw diet induced epigenetic alterations in the mammary tissue of dairy cows.
Feeding a high-concentrate corn straw diet induced epigenetic alterations in the mammary tissue of dairy cows.
复制标题
DOI：
10.1371/journal.pone.0107659
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Yang Y
中科院分区：
文献类型：
--
作者：
Dong G;Qiu M;Ao C;Zhou J;Khas-Erdene;Wang X;Zhang Z;Yang Y
本研究的目的是探讨饲喂高浓度玉米秸秆（HCS）日粮（65%浓缩物+35%玉米秸秆）与低浓度玉米秸秆（LCS）日粮（46%浓缩物+54%玉米秸秆）和低浓度混合饲草（LMF）日粮（46%浓缩物+54%混合饲草）相比，对奶牛乳腺组织表观遗传变化的影响。经产的泌乳中期中国荷斯坦奶牛饲喂这三种饲料中的一种，持续 6 周，同时收集血液样本和乳腺组织样本。分析乳腺动脉和静脉血样本的脂多糖 (LPS) 浓度，同时分析乳腺组织样本的组蛋白 H3 乙酰化以及与脂肪和蛋白质合成相关的特定基因的甲基化。组蛋白的提取和组蛋白 H3 乙酰化的定量显示，与饲喂 LCS 日粮的奶牛相比，饲喂 HCS 日粮的奶牛的乙酰化显着降低。与饲喂 LCS 日粮的奶牛相比，饲喂 HCS 日粮的奶牛乳腺动脉血中的 LPS 浓度显着更高。我们发现组蛋白 H3 乙酰化程度与 LPS 浓度呈负相关。在饲喂 HCS 日粮的奶牛中，与乳脂合成相关的硬脂酰辅酶 A 去饱和酶基因的甲基化增加。相比之下，在饲喂 HCS 日粮的奶牛中，编码信号转导子和转录激活子 5A 的基因的甲基化程度降低，这表明饲喂高浓度玉米秸秆日粮可能会改变奶牛乳腺组织中参与脂肪和蛋白质合成的特定基因的甲基化。饲喂高浓度日粮可能通过影响乳腺血液中不同量的脂多糖的释放，诱导奶牛乳腺组织发生表观遗传变化。
The objective of this study was to investigate the effects of feeding a high-concentrate corn straw (HCS) diet (65% concentrate+35% corn straw) on the epigenetic changes in the mammary tissue of dairy cows in comparison with a low-concentrate corn straw (LCS) diet (46% concentrate+54% corn straw) and with a low-concentrate mixed forage (LMF) diet (46% concentrate+54% mixed forage). Multiparous mid-lactation Chinese Holstein cows were fed one of these three diets for 6 weeks, at which time blood samples and mammary tissue samples were collected. Mammary arterial and venous blood samples were analyzed for lipopolysaccharide (LPS) concentrations while mammary tissue samples were assayed for histone H3 acetylation and the methylation of specific genes associated with fat and protein synthesis. Extraction of histones and quantification of histone H3 acetylation revealed that acetylation was significantly reduced in cows fed the HCS diet, as compared with cows fed the LCS diet. Cows fed the HCS diet had significantly higher LPS concentrations in the mammary arterial blood, as compared with cows fed the LCS diet. We found that the extent of histone H3 acetylation was negatively correlated with LPS concentrations. The methylation of the stearoyl-coenzyme A desaturase gene associated with milk fat synthesis was increased in cows fed the HCS diet. By contrast, methylation of the gene encoding the signal transducer and activator of transcription 5A was reduced in cows fed the HCS diet, suggesting that feeding a high-concentrate corn straw diet may alter the methylation of specific genes involved in fat and protein synthesis in the mammary tissue of dairy cows. Feeding the high-concentrate diet induced epigenetic changes in the mammary tissues of dairy cows, possibly through effecting the release of differing amounts of LPS into the mammary blood.
登录
查看更多内容
影响因子：
1
作者：
Li, S.;Gozho, G. N.;Plaizier, J. C.
通讯作者：
Plaizier, J. C.
影响因子：
7.4
作者：
Kim, JY;Kim, KW;Yoo, JS
通讯作者：
Yoo, JS
影响因子：
3.5
作者：
Ametaj, B. N.;Emmanuel, D. G. V.;Dunn, S. M.
通讯作者：
Dunn, S. M.
影响因子：
3.5
作者：
Keunen, JE;Plaizier, JC;McBride, BW
通讯作者：
McBride, BW
影响因子：
3.5
作者：
Emmanuel, D. G. V.;Madsen, K. L.;Ametaj, B. N.
通讯作者：
Ametaj, B. N.