Mining of the Uncharacterized Cytochrome P450 Genes Involved in Alkaloid Biosynthesis in California Poppy Using a Draft Genome Sequence.
Mining of the Uncharacterized Cytochrome P450 Genes Involved in Alkaloid Biosynthesis in California Poppy Using a Draft Genome Sequence.
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DOI：
10.1093/pcp/pcx210
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发表时间：
2018-02-01
影响因子：
4.9
通讯作者：
Sato F
中科院分区：
文献类型：
--
作者：
Hori K;Yamada Y;Purwanto R;Minakuchi Y;Toyoda A;Hirakawa H;Sato F
陆地植物产生特殊的低分子代谢物，以适应各种环境应激因素，如紫外线辐射、病原体感染、创伤和动物饲养损害。由于逆境的多样性，植物通过特殊的生物合成途径产生各种化学物质，特别是植物特有的生物碱。本研究利用基因组草图序列，通过查询已知的生物合成细胞色素P450(P450)酶编码基因，对加州罂粟中参与异喹啉生物碱(BIA)生物合成的P450基因进行了研究，因为P450是参与植物专化代谢多样化的关键酶。我们的计算机研究表明，所有已鉴定的参与BIA生物合成的酶编码基因都存在于约489Mb的基因组草稿序列中，约占整个基因组(502Mb)的97%。进一步的分析表明，参与BIA生物合成的一些P450家族，即CYP80、CYP82和CYP719家族，在拟南芥基因组中的丰度高于在拟南芥基因组中的丰度，拟南芥是一种不产生偏见的植物。由于CYP82家族基因非常丰富，因此我们通过检测不同植物组织和两个BIA产量不同的细胞系中生物碱积累相关基因的表达，来估计这些基因的功能。进一步的鉴定表明，两个高度同源的P450(CYP82P2和CYP82P3)具有不同底物特异性的10-羟基酶活性。在这里，我们讨论了P450基因的进化以及进一步对编码BIA生物合成相关酶的基因进行基因组挖掘的可能性。
Land plants produce specialized low molecular weight metabolites to adapt to various environmental stressors, such as UV radiation, pathogen infection, wounding and animal feeding damage. Due to the large variety of stresses, plants produce various chemicals, particularly plant species-specific alkaloids, through specialized biosynthetic pathways. In this study, using a draft genome sequence and querying known biosynthetic cytochrome P450 (P450) enzyme-encoding genes, we characterized the P450 genes involved in benzylisoquinoline alkaloid (BIA) biosynthesis in California poppy (Eschscholzia californica), as P450s are key enzymes involved in the diversification of specialized metabolism. Our in silico studies showed that all identified enzyme-encoding genes involved in BIA biosynthesis were found in the draft genome sequence of approximately 489 Mb, which covered approximately 97% of the whole genome (502 Mb). Further analyses showed that some P450 families involved in BIA biosynthesis, i.e. the CYP80, CYP82 and CYP719 families, were more enriched in the genome of E. californica than in the genome of Arabidopsis thaliana, a plant that does not produce BIAs. CYP82 family genes were highly abundant, so we measured the expression of CYP82 genes with respect to alkaloid accumulation in different plant tissues and two cell lines whose BIA production differs to estimate the functions of the genes. Further characterization revealed two highly homologous P450s (CYP82P2 and CYP82P3) that exhibited 10-hydroxylase activities with different substrate specificities. Here, we discuss the evolution of the P450 genes and the potential for further genome mining of the genes encoding the enzymes involved in BIA biosynthesis.
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影响因子：
6.4
作者：
LINSMAIER, EM;SKOOG, F
通讯作者：
SKOOG, F
影响因子：
4.8
作者：
Hagel, Jillian M.;Beaudoin, Guillaume A. W.;Facchini, Peter J.
通讯作者：
Facchini, Peter J.
药用植物博落回基因组为苄基异喹啉生物碱代谢提供新见解
影响因子：
27.5
作者：
Liu, Xiubin;Liu, Yisong;Zeng, Jianguo
通讯作者：
Zeng, Jianguo
影响因子：
3.8
作者：
DEEKNAMKUL, W;TANAHASHI, T;ZENK, MH
通讯作者：
ZENK, MH
影响因子：
7.2
作者：
Liscombe, David K.;Ziegler, Joerg;Facchini, Peter J.
通讯作者：
Facchini, Peter J.