The activity and copy number of mitochondrial DNA in ovine oocytes throughout oogenesis in vivo and during oocyte maturation in vitro.
The activity and copy number of mitochondrial DNA in ovine oocytes throughout oogenesis in vivo and during oocyte maturation in vitro.
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DOI：
10.1093/molehr/gat013
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发表时间：
2013-07
影响因子：
4
通讯作者：
Picton HM
中科院分区：
文献类型：
--
作者：
Cotterill M;Harris SE;Collado Fernandez E;Lu J;Huntriss JD;Campbell BK;Picton HM
线粒体负责ATP的产生，ATP驱动细胞代谢和生物合成过程。这是第一个定量研究在一个大的单排卵物种的卵子发生的所有阶段的mtDNA拷贝数，它包括通过JC 1染色评估的萌发囊泡（GV）和中期II（MII）的卵母细胞的线粒体的活性。在37°C消化1小时后，从绵羊卵巢皮质分离原始至早期有腔卵泡（n = 249），并且所有卵母细胞从其体细胞中解聚。从直径为3- 5 mm的窦状卵泡中吸出成熟囊泡卵母细胞（n = 133），并在体外成熟（IVM）后产生成熟的MII卵母细胞（n = 71）。结果显示，从原始卵泡（605 ± 205，n = 8）到成熟MII期卵母细胞（744633 ± 115799，n = 13; P < 0.05），卵母细胞中mtDNA拷贝数呈递增趋势，但变化幅度不一。在IVM过程中，从GV期到MII期的减数分裂进程中，线粒体活性没有改变（P > 0.05）。在卵子发生过程中观察到的mtDNA拷贝数的增加反映了在卵母细胞生长和成熟过程中协调细胞骨架和细胞质重组所需的ATP需求的变化，以及在排卵前促性腺激素激增后促进成熟卵母细胞减数分裂恢复的需要。
Mitochondria are responsible for the production of ATP, which drives cellular metabolic and biosynthetic processes. This is the first study to quantify the mtDNA copy number across all stages of oogenesis in a large monovulatory species, it includes assessment of the activity of mitochondria in germinal vesicle (GV) and metaphase II (MII) oocytes through JC1 staining. Primordial to early antral follicles (n = 249) were isolated from the sheep ovarian cortex following digestion at 37°C for 1 h and all oocytes were disaggregated from their somatic cells. Germinal vesicle oocytes (n = 133) were aspirated from 3- to 5-mm diameter antral follicles, and mature MII oocytes (n = 71) were generated following in vitro maturation (IVM). The mtDNA copy number in each oocyte was quantified using real-time PCR and showed a progressive, but variable increase in the amount of mtDNA in oocytes from primordial follicles (605 ± 205, n = 8) to mature MII oocytes (744 633 ± 115 799, n = 13; P < 0.05). Mitochondrial activity (P > 0.05) was not altered during meiotic progression from GV to MII during IVM. The observed increase in the mtDNA copy number across oogenesis reflects the changing ATP demands needed to orchestrate cytoskeletal and cytoplasmic reorganization during oocyte growth and maturation and the need to fuel the resumption of meiosis in mature oocytes following the pre-ovulatory gonadotrophin surge.
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影响因子：
6.1
作者：
Jones, A;Van Blerkom, J;Toledo, AA
通讯作者：
Toledo, AA
影响因子：
3.6
作者：
Brevini, TAL;Vassena, R;Gandolfi, F
通讯作者：
Gandolfi, F
影响因子：
1.7
作者：
Cummins, JM;Wakayama, T;Yanagimachi, R
通讯作者：
Yanagimachi, R
影响因子：
5.8
作者：
Oktay, K;Briggs, D;Gosden, RG
通讯作者：
Gosden, RG
影响因子：
6.1
作者：
Barritt, JA;Brenner, CA;Cohen, J
通讯作者：
Cohen, J