Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells.
Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells.
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DOI：
10.1186/ar2459
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发表时间：
2008
影响因子：
4.9
通讯作者：
Tuan RS
中科院分区：
文献类型：
--
作者：
Janjanin S;Djouad F;Shanti RM;Baksh D;Gollapudi K;Prgomet D;Rackwitz L;Joshi AS;Tuan RS
间充质祖细胞（MPCs）是成体组织例如骨髓（BM）中的多能祖细胞。目前BM衍生的MPC的临床应用的挑战包括供体部位发病率和疼痛以及与年龄相关的细胞数量和分化潜力减少相关的低细胞产量，强调需要确定MPC的替代来源。最近，MPC来源多样化;实例包括脂肪、胎盘、脐、骨小梁、软骨和滑膜组织。在目前的工作中，我们报告的存在下，MPC在人类扁桃体组织。我们进行了比较和定量分析的BM-MPCs的贴壁细胞从这个淋巴组织，称为扁桃体衍生的MPCs（T-MPCs）的亚群。通过荧光激活细胞分选分析评估表面标志物的表达。分化潜能的T-MPCs进行了组织化学分析，并通过逆转录-聚合酶链反应的谱系相关标记基因的表达。在体外混合淋巴细胞反应中测定MPCs的免疫抑制特性。表面表位分析显示，T-MPCs对CD 14、CD 31、CD 34和CD 45表达呈阴性，对CD 29、CD 44、CD 90和CD 105表达呈阳性，这是BM-MPCs的特征表型。与BM-MPCs相似，T-MPCs可以被诱导经历成脂分化，并且在较小程度上，成骨和软骨分化。T-MPCs不表达II类主要组织相容性（MHC）抗原，与BM-MPCs相比，T-MPCs具有免疫抑制作用，通过吲哚胺2，3-双加氧酶依赖性机制抑制同种异体T细胞或非特异性促有丝分裂刺激的T细胞增殖。人腭T-MPC代表了祖细胞的新来源，可能适用于基于细胞的治疗。
Mesenchymal progenitor cells (MPCs) are multipotent progenitor cells in adult tissues, for example, bone marrow (BM). Current challenges of clinical application of BM-derived MPCs include donor site morbidity and pain as well as low cell yields associated with an age-related decrease in cell number and differentiation potential, underscoring the need to identify alternative sources of MPCs. Recently, MPC sources have diversified; examples include adipose, placenta, umbilicus, trabecular bone, cartilage, and synovial tissue. In the present work, we report the presence of MPCs in human tonsillar tissue. We performed comparative and quantitative analyses of BM-MPCs with a subpopulation of adherent cells isolated from this lymphoid tissue, termed tonsil-derived MPCs (T-MPCs). The expression of surface markers was assessed by fluorescent-activated cell sorting analysis. Differentiation potential of T-MPCs was analyzed histochemically and by reverse transcription-polymerase chain reaction for the expression of lineage-related marker genes. The immunosuppressive properties of MPCs were determined in vitro in mixed lymphocyte reactions. Surface epitope analysis revealed that T-MPCs were negative for CD14, CD31, CD34, and CD45 expression and positive for CD29, CD44, CD90, and CD105 expression, a characteristic phenotype of BM-MPCs. Similar to BM-MPCs, T-MPCs could be induced to undergo adipogenic differentiation and, to a lesser extent, osteogenic and chondrogenic differentiation. T-MPCs did not express class II major histocompatibility (MHC) antigens, and in a similar but less pronounced manner compared with BM-MPCs, T-MPCs were immunosuppressive, inhibiting the proliferation of T cells stimulated by allogeneic T cells or by non-specific mitogenic stimuli via an indoleamine 2,3-dioxygenase-dependent mechanism. Human palatine T-MPCs represent a new source of progenitor cells, potentially applicable for cell-based therapies.
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影响因子：
2.6
作者：
Bartholomew, A;Sturgeon, C;Hoffman, R
通讯作者：
Hoffman, R
影响因子：
4.9
作者：
Djouad F;Bony C;Häupl T;Uzé G;Lahlou N;Louis-Plence P;Apparailly F;Canovas F;Rème T;Sany J;Jorgensen C;Noël D
通讯作者：
Noël D
影响因子：
20.3
作者：
Aggarwal, S;Pittenger, MF
通讯作者：
Pittenger, MF
影响因子：
20.3
作者：
Beyth, S;Borovsky, Z;Rachmilewitz, J
通讯作者：
Rachmilewitz, J
影响因子：
5.2
作者：
Baxter, MA;Wynn, RF;Bellantuono, I
通讯作者：
Bellantuono, I