Bacterial cellulose spheroids as building blocks for 2D and 3D engineered living materials
Bacterial cellulose spheroids as building blocks for 2D and 3D engineered living materials
复制标题
细菌纤维素球体作为 2D 和 3D 工程生活材料的构建模块
DOI：
10.1101/2020.05.11.088138
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Caro-Astorga J
中科院分区：
文献类型：
--
作者：
Caro-Astorga J
基于细菌纤维素（BC）的工程生物材料（ELMs）为廉价生产的材料提供了一条有前途的途径，这种材料可以被编程为具有基因编码功能。在这里，我们探索了如何利用醋酸细菌偶尔产生的毫米级纤维素球（我们称之为BC球体）制造elm。我们定义了一种可重复的方案来生产BC球体，并展示了它们作为构建块在2D和3D形状中生长elm的潜力。这些BC球体可以被基因功能化，并用作制造和生长基于BC的elm的方法来设计。我们进一步展示了BC球体用于BC材料的修复和再生，并测量了材料中产生BC的细菌随时间的存活率。这项工作推进了我们对BC球体形成的理解，并展示了它们在创造和修复工程生物材料方面的潜力。
Engineered living materials (ELMs) based on bacterial cellulose (BC) offer a promising avenue for cheap-to-produce materials that can be programmed with genetically encoded functionalities. Here we explore how ELMs can be fabricated from millimetre-scale balls of cellulose occasionally produced byAcetobacteriaceaspecies, which we call BC spheroids. We define a reproducible protocol to produce BC spheroids and demonstrate their potential for use as building blocks to grow ELMs in 2D and 3D shapes. These BC spheroids can be genetically functionalized and used as the method to make and grow patterned BC-based ELMs to design. We further demonstrate the use of BC spheroids for the repair and regeneration of BC materials, and measure the survival of the BC-producing bacteria in the material over time. This work forwards our understanding of BC spheroid formation and showcases their potential for creating and repairing engineered living materials.
影响因子：
3.7
作者：
Limoli DH;Jones CJ;Wozniak DJ
通讯作者：
Wozniak DJ
影响因子：
41.2
作者：
Gilbert, Charlie;Tang, Tzu-Chieh;Ellis, Tom
通讯作者：
Ellis, Tom