Prevalence of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae and Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae in China Determined via Mouse Lethality Tests.
Prevalence of Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae and Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae in China Determined via Mouse Lethality Tests.
复制标题
通过小鼠致死试验确定中国耐碳青霉烯类高毒力肺炎克雷伯菌和高毒力耐碳青霉烯类肺炎克雷伯菌的流行情况
DOI：
10.3389/fcimb.2022.882210
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发表时间：
2022
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
--
作者：
目的了解耐碳青霉烯类肺炎克雷伯菌高毒力株（CR-HvKP）和耐碳青霉烯类肺炎克雷伯菌高毒力株（Hv-CRKP）的流行病学特征。共436 K 2017年1月至2018年2月期间，从中国大陆7家医院收集肺炎菌株。分析了序列类型、血清型、耐药性和毒力基因。此外，还进行了绳试验、胶囊染色、高碘酸希夫染色、适合度分析、定量实时PCR和小鼠致死率试验。采用分子组合筛选推定的bla KPC（+）-HvKP和Hv-bla KPC（+）-KP，随后进行小鼠致死试验确认。毒力基因的检出率从c-rmpA（0.0%）到entB（100.0%）不等。根据分子生物学鉴定标准，HvKP、bla KPC（+）-KP、bla KPC（+）-HvKP和Hv-bla KPC（+）-KP分别为127、186、9和26株。小鼠致死性试验证实了2株bla KPC（+）-HvKP菌株（JS 184和TZ 20），无Hv-bla KPC（+）-KP。JS 184为K2血清型，荚膜薄，外泌多糖阳性，线试验阳性。TZ 20血清型为K20型，荚膜薄，胞外多糖阴性，线试验阴性。与阳性对照NTUH-K2044相比，确认了JS 184和TZ 20的相同galF表达和生长曲线。CR-HvKP和Hv-CRKP的分子测定与小鼠致死试验相比存在明显的偏倚。CR-HvKP的确切患病率小于1.0%，Hv-CRKP的确切患病率更低。
To investigate the epidemiology of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-HvKP) and hypervirulent carbapenem-resistant Klebsiella pneumoniae (Hv-CRKP). Totally 436 K. pneumoniae strains were collected from 7 hospitals in mainland China between 2017.01 and 2018.02. Sequence types, serotypes, antimicrobial-resistance and virulence genes were analyzed. Additionally, string test, capsule stain, Periodic Acid Schiff stain, fitness analysis, quantitative real-time PCR and mouse lethality test were also performed. Molecular combinations were used to screen putative bla KPC(+)-HvKP and Hv-bla KPC(+)-KP, followed by the confirmation of mouse lethality test. Diverse detection rates were found for the virulence genes, ranging from c-rmpA (0.0%) to entB (100.0%). According to the molecular criteria, 127, 186, 9 and 26 strains were putatively denoted as HvKP, bla KPC(+)-KP, bla KPC(+)-HvKP and Hv-bla KPC(+)-KP. Mouse lethality test confirmed 2 bla KPC(+)-HvKP strains (JS184 and TZ20) and no Hv-bla KPC(+)-KP. JS184 showed K2 serotype, thin capsule, positive exopolysaccharid and string test. TZ20 presented K20 serotype, thin capsule, negative exopolysaccharide and string test. Compared with the positive control NTUH-K2044, equal galF expression and growth curves were confirmed for JS184 and TZ20. Molecular determination of CR-HvKP and Hv-CRKP brings remarkable bias compared with mouse lethality test. The exact prevalence of CR-HvKP is less than 1.0%, which of Hv-CRKP is much lower.
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影响因子：
3.1
作者：
MIZUTA, K;OHTA, M;KATO, N
通讯作者：
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作者：
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影响因子：
2.6
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