A paradox: rapid evolution rates of germline-limited sequences are associated with conserved patterns of rearrangements in cryptic species of Chilodonella uncinata (Protista, Ciliophora)
A paradox: rapid evolution rates of germline-limited sequences are associated with conserved patterns of rearrangements in cryptic species of Chilodonella uncinata (Protista, Ciliophora)
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一个悖论：种系限制序列的快速进化速率与 Chilodonella uncinata（Protista、Ciliophora）的隐秘物种中的保守重排模式相关
DOI：
10.1007/s11427-018-9333-1
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发表时间：
2018-07
期刊：
影响因子：
--
通讯作者：
Gao Feng 唯一通讯
中科院分区：
文献类型：
--
作者：
Zhang Tengteng;Wang Chundi;Katz Laura A.;Gao Feng 唯一通讯
一些最极端的全基因组重排发现于纤毛虫中，纤毛虫在每个细胞/生物体中都具有生殖细胞微核和体细胞大核。在大核发育过程中，发生了一系列DNA重排事件，包括DNA消除、染色体断裂、基因重排和替代加工。为了评估Chilodonella uncinata不同神秘物种大核和种系限制序列的分子进化，我们对USA-SC 2株的微核和大核基因组中的肌动蛋白、α-微管蛋白和β-微管蛋白基因进行了表征，并将其与其他菌株（即神秘物种）进行了比较。主要结果有：（1）隐种C.具钩的;（ii）相反，种系限制区是高度不同的序列和长度之间的神秘物种;（iii）指针移动是频繁的神秘物种。我们推测，指针序列可能作为保守的大核注定序列和快速进化的内部消除序列之间的缓冲。结合以往的研究数据表明，基因重排的可塑性在不同群体的纤毛虫和添加到不断增长的数据基因组重排在物种分化中的作用。
Some of the most extreme genome wide rearrangements are found in ciliates, which are unique in possessing both germline micronucleus and somatic macronucleus in every cell/organism. A series of DNA rearrangement events, including DNA elimination, chromosomal fragmentation, gene unscrambling and alternative processing, happen during macronuclear development. To assess the molecular evolution of macronuclear and germline-limited sequences in different cryptic species of Chilodonella uncinata, we characterized the actin, α-tubulin and β-tubulin genes in the micronucleus and macronucleus genomes of USA-SC2 strain and compared them with other strains (i.e. cryptic species). Three main results are: (i) rearrangement patterns between germline and soma are conserved for each gene among the cryptic species of C. uncinata; (ii) in contrast, the germline-limited regions are highly divergent in sequence and length among the cryptic species; (iii) pointer shifting is frequent among the cryptic species. We speculate that pointer sequences may serve as the buffer between the conserved macronuclear destined sequences and rapidly-evolving internal eliminated sequences. The data combined with previous studies demonstrate the plasticity of gene rearrangement among different groups of ciliates and add to the growing data for the role of genome rearrangements in species differentiation.
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影响因子：
64.8
作者：
Joron, Mathieu;Frezal, Lise;Jones, Robert T.;Chamberlain, Nicola L.;Lee, Siu F.;Haag, Christoph R.;Whibley, Annabel;Becuwe, Michel;Baxter, Simon W.;Ferguson, Laura;Wilkinson, Paul A.;Salazar, Camilo;Davidson, Claire;Clark, Richard;Quail, Michael A.;Beasley, Helen;Glithero, Rebecca;Lloyd, Christine;Sims, Sarah;Jones, Matthew C.;Rogers, Jane;Jiggins, Chris D.;Ffrench-Constant, Richard H.
通讯作者：
Ffrench-Constant, Richard H.
影响因子：
4.4
作者：
Ricard, Guenola;de Graaf, Rob M.;Dutilh, Bas E.;Duarte, I.;van Alen, Theo A.;van Hoek, Angela H. A. M.;Boxma, Brigitte;van der Staay, Georg W. M.;Moon-van der Staay, Seung Yeo;Chang, Wei-Jen;Landweber, Laura F.;Hackstein, Johannes H. P.;Huynen, Martijn A.
通讯作者：
Huynen, Martijn A.
影响因子：
9.8
作者：
Stockdale C;Swiderski MR;Barry JD;McCulloch R
通讯作者：
McCulloch R
影响因子：
3.9
作者：
R. Zufall;Mariel Sturm;Brian C. Mahon
通讯作者：
R. Zufall;Mariel Sturm;Brian C. Mahon
影响因子：
10.7
作者：
Robinson, T.;Katz, L. A.
通讯作者：
Katz, L. A.