Oral delivery of human biopharmaceuticals, autoantigens and vaccine antigens bioencapsulated in plant cells.
Oral delivery of human biopharmaceuticals, autoantigens and vaccine antigens bioencapsulated in plant cells.
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DOI：
10.1016/j.addr.2012.10.005
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发表时间：
2013-06-15
影响因子：
16.1
通讯作者：
Daniell, Henry
中科院分区：
文献类型：
--
作者：
Kwon, Kwang-Chul;Verma, Dheeraj;Singh, Nameirakpam D.;Herzog, Roland;Daniell, Henry
关键词：
在每年进行的120亿次注射中，不安全的分娩导致超过2000万例感染和超过1亿例反应。在一个新兴的新概念中，表达疫苗抗原/生物药物的冻干植物细胞（莴苣）在胃中受到保护，不受酸/酶的影响，但当植物细胞壁被肠道微生物消化时，释放到免疫或血液循环系统。在引发后口服递送时，生物包封在植物细胞中的疫苗抗原赋予粘膜和全身免疫力以及针对细菌、病毒或原生动物病原体或毒素攻击的保护。口服自身抗原通过产生耐受性，对1型糖尿病和血友病的并发症有效。口服递送在植物细胞中表达的胰岛素原或exendin-4类似于注射来调节血糖水平。因此，这种新平台提供了一种低成本的替代方案，通过消除灭活的病原体、昂贵的纯化、冷藏/运输和无菌注射来递送不同的治疗性蛋白质，以对抗感染性或遗传性疾病。
Among 12 billion injections administered annually, unsafe delivery leads to >20 million infections and >100 million reactions. In an emerging new concept, freeze-dried plant cells (lettuce) expressing vaccine antigens/biopharmaceuticals are protected in the stomach from acids/enzymes but are released to the immune or blood circulatory system when plant cell walls are digested by microbes that colonize the gut. Vaccine antigens bioencapsulated in plant cells upon oral delivery after priming, conferred both mucosal and systemic immunity and protection against bacterial, viral or protozoan pathogens or toxin challenge. Oral delivery of autoantigens was effective against complications of type 1diabetes and hemophilia, by developing tolerance. Oral delivery of proinsulin or exendin-4 expressed in plant cells regulated blood glucose levels similar to injections. Therefore, this new platform offers a low cost alternative to deliver different therapeutic proteins to combat infectious or inherited diseases by eliminating inactivated pathogens, expensive purification, cold storage/transportation and sterile injections.
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影响因子：
20.3
作者：
Cao, Ou;Armstrong, Elina;Herzog, Roland W.
通讯作者：
Herzog, Roland W.
影响因子：
1.5
作者：
Angov, E;Aufiero, BM;Lyon, JA
通讯作者：
Lyon, JA
影响因子：
5.2
作者：
Aide, Pedro;Bassat, Quique;Alonso, Pedro L.
通讯作者：
Alonso, Pedro L.
影响因子：
10.8
作者：
Boucaud, A;Garrigue, MA;Patat, F
通讯作者：
Patat, F
影响因子：
4.9
作者：
Cui, Fuying;He, Chunbai;Yin, Chunhua
通讯作者：
Yin, Chunhua