Microneedle patch delivery to the skin of virus-like particles containing heterologous M2e extracellular domains of influenza virus induces broad heterosubtypic cross-protection.
Microneedle patch delivery to the skin of virus-like particles containing heterologous M2e extracellular domains of influenza virus induces broad heterosubtypic cross-protection.
复制标题
DOI：
10.1016/j.jconrel.2015.05.278
复制
发表时间：
2015-07-28
期刊：
影响因子：
--
通讯作者：
Kang SM
中科院分区：
文献类型：
--
作者：
Kim MC;Lee JW;Choi HJ;Lee YN;Hwang HS;Lee J;Kim C;Lee JS;Montemagno C;Prausnitz MR;Kang SM
一种广泛的交叉保护性流感疫苗，可以通过无痛的自我免疫方法管理，将是一个潜在的普遍的大规模疫苗接种策略的价值。本研究开发了一种微创微针（MN）贴片，用于皮肤接种含有流感病毒异源M2胞外（M2 e）结构域（M2 e5 x VLP）的病毒样颗粒，作为不含佐剂的通用候选疫苗。M2 e5 x VLP涂层微针的稳定性在室温下可保持8周，而不会丧失M2 e抗原性和免疫原性。MN皮肤免疫诱导了强烈的体液和粘膜M2 e抗体应答，并赋予针对H1N1、H3 N2和H5 N1亚型流感病毒攻击的交叉保护。此外，M2 e5 x VLP MN皮肤接种诱导的1型辅助性T细胞应答，如IgG 2a同种型抗体和IFN-γ产生细胞的水平高于常规肌内注射。皮肤递送M2 e5 x VLP MN疫苗的这些潜在的免疫学和物流优势可以提供有希望的方法来开发易于施用的通用流感疫苗。
A broadly cross-protective influenza vaccine that can be administrated by a painless self-immunization method would be a value as a potential universal mass vaccination strategy. This study developed a minimally-invasive microneedle (MN) patch for skin vaccination with virus-like particles containing influenza virus heterologous M2 extracellular (M2e) domains (M2e5x VLPs) as a universal vaccine candidate without adjuvants. The stability of M2e5x VLP-coated microneedles was maintained for 8 weeks at room temperature without losing M2e antigenicity and immunogenicity. MN skin immunization induced strong humoral and mucosal M2e antibody responses and conferred cross-protection against heterosubtypic H1N1, H3N2, and H5N1 influenza virus challenges. In addition, M2e5x VLP MN skin vaccination induced T-helper type 1 responses such as IgG2a isotype antibodies and IFN-γ producing cells at higher levels than those by conventional intramuscular injection. These potential immunological and logistic advantages for skin delivery of M2e5x VLP MN vaccines could offer a promising approach to develop an easy-to-administer universal influenza vaccine.
登录
查看更多内容
人类感染新型禽源甲型流感 (H7N9) 病毒。
影响因子：
158.5
作者：
Gao, Rongbao;Cao, Bin;Shu, Yuelong
通讯作者：
Shu, Yuelong
影响因子：
5.5
作者：
Eliasson, Dubravka Grdic;El Bakkouri, Karim;Lycke, Nits
通讯作者：
Lycke, Nits
影响因子：
5.4
作者：
Jayasekera, Jerome P.;Moseman, E. Ashley;Carroll, Michael C.
通讯作者：
Carroll, Michael C.
影响因子：
5.5
作者：
Ernst, William A.;Kim, Hyung J.;Fujii, Gary
通讯作者：
Fujii, Gary
影响因子：
64.8
作者：
Neumann, Gabriele;Noda, Takeshi;Kawaoka, Yoshihiro
通讯作者：
Kawaoka, Yoshihiro