Expression of doublecortin, a neuronal migration protein, in unipolar brush cells of the vestibulocerebellum and dorsal cochlear nucleus of the adult rat.
Expression of doublecortin, a neuronal migration protein, in unipolar brush cells of the vestibulocerebellum and dorsal cochlear nucleus of the adult rat.
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DOI：
10.1016/j.neuroscience.2011.12.013
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发表时间：
2012-01-27
期刊：
影响因子：
3.3
通讯作者：
Baizer, J. S.
中科院分区：
文献类型：
--
作者：
Manohar, S.;Paolone, N. A.;Bleichfeld, M.;Hayes, S. H.;Salvi, R. J.;Baizer, J. S.
双皮质素（DCX）是一种微管相关蛋白，对神经元迁移和大脑皮层发育至关重要。在成人中，它在室下和颗粒下区的新生神经元中表达，但不在大脑皮层的成熟神经元中表达。相比之下，小脑中细胞的神经发生和神经元迁移持续到出生后的早期;一类小脑中间神经元，单极刷细胞（UBC）的迁移可能会持续到成年。为了探索成年小脑中持续神经元迁移的可能性，对成年（3-16个月大）Sprague道利大鼠的脑干和小脑的紧密间隔切片进行DCX免疫标记。DCX免疫反应阳性神经元主要分布于前庭小脑颗粒细胞层，以过渡区（tz）、绒球（FL）与腹侧副绒球（PFL）之间的区域以及耳蜗背核（DCN）最为密集。这些DCX-ir细胞具有单极刷状细胞（UBC）的形态学外观，具有椭圆形胞体和单个树突以“刷”结束。有许多DCX与Eps 8或calretinin，UBC标记物共定位的例子。我们还确定了沿着第四脑室及其侧隐窝的DCX-ir元素，这些元素标记了胞体，但缺乏UBC的树突状结构特征。在附近的白色物质中观察到标记的UBC。这些结果表明，可能有持续的神经发生和/或迁移的UBC在成人。另一种可能性是UBC即使在迁移和成熟后仍保持DCX表达，这反映了DCX在成人神经元可塑性中的作用以及在迁移中的发育作用。
Doublecortin (DCX) is a microtubule associated protein that is critical for neuronal migration and the development of the cerebral cortex. In the adult, it is expressed in newborn neurons in the subventricular and subgranular zones but not in the mature neurons of the cerebral cortex. By contrast, neurogenesis and neuronal migration of cells in the cerebellum continue into early postnatal life; migration of one class of cerebellar interneuron, unipolar brush cells (UBCs), may continue into adulthood. To explore the possibility of continued neuronal migration in the adult cerebellum, closely spaced sections through the brainstem and cerebellum of adult (3–16 months old) Sprague Dawley rats were immunolabeled for DCX. Neurons immunoreactive (ir) to DCX were present in the granular cell layer of the vestibulocerebellum, most densely in the transition zone (tz), the region between the flocculus (FL) and ventral paraflocculus (PFL), as well as in the dorsal cochlear nucleus (DCN). These DCX-ir cells had the morphological appearance of unipolar brush cells (UBCs) with oval somata and a single dendrite ending in a “brush.” There were many examples of colocalization of DCX with Eps8 or calretinin, UBC markers. We also identified DCX-ir elements along the fourth ventricle and its lateral recess that had labeled somata but lacked the dendritic structure characteristic of UBCs. Labeled UBCs were seen in nearby white matter. These results suggest that there may be continued neurogenesis and/or migration of UBCs in the adult. Another possibility is that UBCs maintain DCX expression even after migration and maturation, reflecting a role of DCX in adult neuronal plasticity in addition to a developmental role in migration.
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影响因子：
2
作者：
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通讯作者：
Mugnaini, E
影响因子：
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