Microtubule-mediated centrosome motility and the positioning of cleavage furrows in multinucleate myosin II-null cells.
Microtubule-mediated centrosome motility and the positioning of cleavage furrows in multinucleate myosin II-null cells.
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微管介导的中心体运动和多核肌球蛋白 II 缺失细胞中裂解沟的定位。
DOI：
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发表时间：
1998
影响因子：
4
通讯作者：
Günther Gerisch
中科院分区：
文献类型：
--
作者：
R. Neujahr;R. Albrecht;Jana Köhler;M. Matzner;J. Schwartz;M. Westphal;Günther Gerisch
为了研究有丝分裂、有丝分裂后和分裂间期细胞中中心体的运动以及微管与细胞皮层的相互作用，用绿色荧光蛋白标记盘基网柄藻（Dictyosteelicdiscoideum）的（α）-微管蛋白。由肌球蛋白II无效突变体形成的多核细胞被证明是特别适合于分析由微管系统控制的卵裂沟形成。在分裂后期，有丝分裂器与细胞皮质对接后，细胞表面被激活，在中心体发出的微管星形细胞顶部形成皱褶。裂沟起始于星状花序之间的空间，与纺锤体的位置无关。一旦开始，沟扩大为深折叠，没有持续连接到微管系统。单侧沟的出现表明在网骨藻中有一个封闭的收缩环，用于胞质分裂。在多核细胞胞质分裂的进展强调了蛋白质的重要性，而不是肌球蛋白II在指定一个卵裂沟。中心体运动的分析表明，负端定向马达蛋白，可能是细胞质动力蛋白，在施加牵引力的指导微管连接中心体与细胞皮质的主要作用。
To study centrosome motility and the interaction of microtubules with the cell cortex in mitotic, post-mitotic and interphase cells, (alpha)-tubulin was tagged in Dictyostelium discoideum with green fluorescent protein. Multinucleate cells formed by myosin II-null mutants proved to be especially suited for the analysis of the control of cleavage furrow formation by the microtubule system. After docking of the mitotic apparatus onto the cell cortex during anaphase, the cell surface is activated to form ruffles on top of the asters of microtubules that emanate from the centrosomes. Cleavage furrows are initiated at spaces between the asters independently of the positions of spindles. Once initiated, the furrows expand as deep folds without a continued connection to the microtubule system. Occurrence of unilateral furrows indicates that a closed contractile ring is dispensable for cytokinesis in Dictyostelium. The progression of cytokinesis in the multinucleate cells underlines the importance of proteins other than myosin II in specifying a cleavage furrow. The analysis of centrosome motility suggests a major role for a minus-end directed motor protein, probably cytoplasmic dynein, in applying traction forces on guiding microtubules that connect the centrosome with the cell cortex.
影响因子：
3.3
作者：
Cao, LG;Wang, YL
通讯作者：
Wang, YL
影响因子：
11.4
作者：
MANSTEIN, DJ;TITUS, MA;SPUDICH, JA
通讯作者：
SPUDICH, JA
影响因子：
56.9
作者：
DELOZANNE, A;SPUDICH, JA
通讯作者：
SPUDICH, JA