Quality metrics for stem cell-derived cardiac myocytes.
Quality metrics for stem cell-derived cardiac myocytes.
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DOI：
10.1016/j.stemcr.2014.01.015
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发表时间：
2014-03-11
影响因子：
5.9
通讯作者：
Parker, Kevin Kit
中科院分区：
文献类型：
--
作者：
Sheehy, Sean P.;Pasqualini, Francesco;Grosberg, Anna;Park, Sung Jin;Aratyn-Schaus, Yvonne;Parker, Kevin Kit
干细胞制造方法的进步使得以工业规模生产干细胞衍生的心肌细胞用于体外肌肉生理学研究目的成为可能。虽然FDA规定的质量保证指标解决了基于干细胞的产品生产中的安全问题，但目前还没有用于评估干细胞衍生的肌细胞功能的标准化指南。因此，目前尚不清楚市场上的各种干细胞衍生的肌细胞系是否表现相似，或者它们中的任何一种是否准确地概括了天然心肌细胞的特征。我们提出了一个多参数的质量评估量规，其中遗传，结构，电生理和收缩测量与比较，这些测量值是心室肌细胞表型的代表。我们证明了这一程序使用市售的，大量生产的小鼠胚胎干细胞和诱导多能干细胞衍生的肌细胞相比，在耦合的体外试验中的新生小鼠心室肌细胞靶表型。干细胞来源的心肌细胞形态和功能的综合评价干细胞来源的心肌细胞质量保证的多因素规则干细胞来源的心肌细胞和分离的心肌细胞的直接比较将多能干细胞分化为心肌细胞的技术已经发展到这样的细胞现在可以大规模生产用于商业销售。然而，几乎没有努力确保这些大量生产的心肌细胞表现出健康成熟心肌的功能性能特征。帕克及其同事开发了一种用于干细胞衍生的心肌细胞的组织工程试验床，该试验床允许对心肌细胞的形式和功能进行多因素评估，以及与目标心脏表型进行比较。
Advances in stem cell manufacturing methods have made it possible to produce stem cell-derived cardiac myocytes at industrial scales for in vitro muscle physiology research purposes. Although FDA-mandated quality assurance metrics address safety issues in the manufacture of stem cell-based products, no standardized guidelines currently exist for the evaluation of stem cell-derived myocyte functionality. As a result, it is unclear whether the various stem cell-derived myocyte cell lines on the market perform similarly, or whether any of them accurately recapitulate the characteristics of native cardiac myocytes. We propose a multiparametric quality assessment rubric in which genetic, structural, electrophysiological, and contractile measurements are coupled with comparison against values for these measurements that are representative of the ventricular myocyte phenotype. We demonstrated this procedure using commercially available, mass-produced murine embryonic stem cell- and induced pluripotent stem cell-derived myocytes compared with a neonatal mouse ventricular myocyte target phenotype in coupled in vitro assays. Comprehensive evaluation of stem cell-derived cardiac myocyte form and function A multifactorial rubric for stem cell-derived myocyte quality assurance Direct comparison of stem cell-derived myocytes and isolated cardiac myocytes Techniques for differentiating pluripotent stem cells into cardiac myocytes have advanced to the point that such cells are now mass produced for commercial distribution. However, little effort has been made to ensure that these mass-produced myocytes exhibit the functional performance characteristics of the healthy, mature myocardium. Parker and colleagues have a developed a tissue engineering test bed for stem cell-derived myocytes that allows multifactorial evaluation of cardiac myocyte form and function, as well as comparison against a target cardiac phenotype.
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影响因子：
6.1
作者：
Grosberg A;Alford PW;McCain ML;Parker KK
通讯作者：
Parker KK
影响因子：
14
作者：
Feinberg, Adam W.;Alford, Patrick W.;Jin, Hongwei;Ripplinger, Crystal M.;Werdich, Andreas A.;Sheehy, Sean P.;Grosberg, Anna;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit
DOI：
10.1073/pnas.1304913110
发表时间：
2013-06-11
影响因子：
11.1
作者：
McCain, Megan L.;Sheehy, Sean P.;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit
影响因子：
5.5
作者：
Fedorov, Vadim V.;Lozinsky, Ilya T.;Efimov, Igor R.
通讯作者：
Efimov, Igor R.
影响因子：
3.5
作者：
Rezakhaniha, R.;Agianniotis, A.;Stergiopulos, N.
通讯作者：
Stergiopulos, N.