Effect of claudin 1 on cell proliferation, migration and apoptosis in human cervical squamous cell carcinoma.
Effect of claudin 1 on cell proliferation, migration and apoptosis in human cervical squamous cell carcinoma.
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Claudin 1对人宫颈鳞癌细胞增殖、迁移和凋亡的影响
DOI：
10.3892/or.2020.7889
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发表时间：
2021-03
期刊：
影响因子：
4.2
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Shen Z;Song W;Qian L;Zhu J;Li Y;Li M;Zhang T;Zhao W;Zhou Y;Yang X
Claudin 1是Claudin蛋白家族的成员，在紧密连接中起重要作用。在包括乳腺癌和病毒感染在内的几种疾病中都发现了claudin 1表达水平的升高或降低。然而，claudin 1在宫颈癌中的作用仍有争议。本研究旨在探讨claudin 1在不同人宫颈癌细胞系中的生物学功能。首先，采用慢病毒转导法建立稳定过表达或低表达claudin 1的SiHa和ME-180细胞，并通过逆转录定量PCR和western blot分析检测mRNA和蛋白水平。随后，采用标准方案进行了体外细胞增殖、集落形成和迁移实验，证明claudin 1能够抑制SiHa和ME-180细胞的细胞增殖和迁移。通过流式细胞术和western blotting检测细胞周期和凋亡，结果显示claudin 1抑制细胞周期进程，促进细胞凋亡。为了进一步验证claudin 1是否参与了体内肿瘤的生长，我们通过注射过表达claudin 1的SiHa细胞在胸腺小鼠体内建立了异种移植肿瘤，发现claudin 1在体内可以抑制肿瘤的生长。此外，在the cancer Genome Atlas中分析了不同类型癌症中claudin 1表达与预后的关系。总之，本研究结果表明，claudin 1可能在宫颈鳞状细胞癌中具有抗肿瘤作用，可能作为潜在的治疗靶点具有价值。
Claudin 1 is a member of the claudin protein family that serves an important role in tight junctions. Increased or decreased expression levels of claudin 1 are found in several diseases, including breast cancer and viral infections. However, the function of claudin 1 in cervical cancer remains controversial. The aim of the present study was to investigate the biological functions of claudin 1 in different human cervical cancer cell lines. First, SiHa and ME-180 cells with stable claudin 1 overexpression or knockdown were established using lentiviral transduction, and the mRNA and protein levels were measured via reverse transcription-quantitative PCR and western blot analysis. Subsequently, cell proliferation, colony formation and migration experiments were performed in vitro using standard protocols, demonstrating that claudin 1 was able to inhibit cell proliferation and migration in both SiHa and ME-180 cells. Furthermore, cell cycle and apoptosis were detected via flow cytometry and western blotting, and the results revealed that claudin 1 inhibited cell cycle progression and promoted apoptosis. To further verify whether claudin 1 was involved in tumor growth in vivo, xenograft tumors were established in athymic mice via injecting SiHa cells overexpressing claudin 1, which was found to decrease tumor growth in vivo. Furthermore, the association between claudin 1 expression and prognosis was analyzed in different types of cancer in The Cancer Genome Atlas. Overall, the findings of the present study revealed that claudin 1 may serve an antitumor role in cervical squamous cell carcinoma and may be of value as a potential therapeutic target.
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影响因子：
4
作者：
Calder, Michele D.;Edwards, Nicole A.;Watson, Andrew J.
通讯作者：
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影响因子：
3.4
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影响因子：
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作者：
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通讯作者：
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影响因子：
12.7
作者：
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通讯作者：
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