Reconstructing virus structures from nanometer to near-atomic resolutions with cryo-electron microscopy and tomography.
Reconstructing virus structures from nanometer to near-atomic resolutions with cryo-electron microscopy and tomography.
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DOI：
10.1007/978-1-4614-0980-9_4
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发表时间：
2012
影响因子：
--
通讯作者：
Chiu, Wah
中科院分区：
文献类型：
--
作者：
Chang, Juan;Liu, Xiangan;Rochat, Ryan H.;Baker, Matthew L.;Chiu, Wah
在过去的几十年里，单粒子冷冻电子显微镜（cryo-EM）取得了巨大的进步。该领域已经成熟到可以为二十面体病毒生成近原子分辨率的密度图而不需要结晶的程度。与此同时，通过采用单粒子cryo-EM或cryo-electron tomography（cryo-ET），在确定病毒中非二十面体排列蛋白质的结构方面取得了实质性进展。这门课程中隐含的是新一代电子冷冻显微镜的可用性和对生成这些地图和模型至关重要的计算工具的发展。这种方法使结构生物学家能够更详细地分析处于不同形态发生和生化状态的病毒颗粒的结构。此外，冷冻、水合细胞在被病毒感染过程中的电子成像也为“原位”研究病毒结构开辟了新途径。在这里，我们提出了常用的技术用于获取和处理cryo-EM和cryo-ET数据，并讨论它们对结构病毒学的影响，现在和将来。
The past few decades have seen tremendous advances in single particle electron cryo-microscopy (cryo-EM). The field has matured to the point that near-atomic resolution density maps can be generated for icosahedral viruses without the need for crystallization. In parallel, substantial progress has been made in determining the structures of non-icosahedrally arranged proteins in viruses by employing either single particle cryo-EM or cryo-electron tomography (cryo-ET). Implicit in this course has been the availability of a new generation of electron cryo-microscopes and the development of the computational tools that are essential for generating these maps and models. This methodology has enabled structural biologists to analyze structures in increasing detail for virus particles that are in different morphogenetic and biochemical states. Furthermore, electron imaging of frozen, hydrated cells, in the process of being infected by viruses, has also opened up a new avenue for studying virus structures “in situ”. Here we present the common techniques used to acquire and process cryo-EM and cryo-ET data and discuss their implications for structural virology both now and in the future.
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影响因子：
64.8
作者：
Bottcher, B;Wynne, SA;Crowther, RA
通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1098/rstb.2005.1803
发表时间：
2006-03-29
影响因子：
6.3
作者：
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通讯作者：
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影响因子：
3
作者：
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通讯作者：
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影响因子：
3
作者：
Amat, Fernando;Moussavi, Farshid;Horowitz, Mark
通讯作者：
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