The BAM complex subunit BamE (SmpA) is required for membrane integrity, stalk growth and normal levels of outer membrane {beta}-barrel proteins in Caulobacter crescentus.
The BAM complex subunit BamE (SmpA) is required for membrane integrity, stalk growth and normal levels of outer membrane {beta}-barrel proteins in Caulobacter crescentus.
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BAM 复合体亚基 BamE (SmpA) 是新月柄杆菌膜完整性、茎生长和外膜 {β}-桶蛋白正常水平所必需的。
DOI：
10.1099/mic.0.035055-0
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Bowers,LisaM
中科院分区：
文献类型：
--
作者：
Ryan,KathleenR;Taylor,JamesA;Bowers,LisaM
革兰氏阴性菌的外膜是一个重要的隔室，含有特定的脂质和蛋白质补充，构成保护性的选择性渗透屏障。外膜β-桶蛋白通过含有脂蛋白BamE的必需异源寡聚BAM复合物组装到膜中。我们已经确定了一个同源的BamE，由CC 1365编码，这是位于外膜的茎α-proteobacteriumCaulobacter crescentus。BamE与已知参与外膜蛋白组装的其他细菌中的同源蛋白BamA（CC 1915）、BamB（CC 1653）和BamD（CC 1984）相关联。缺乏BamE的柄杆菌细胞在丰富培养基中生长缓慢，并且对阴离子去污剂、一些抗生素和热暴露高度敏感，这表明突变体的膜完整性受到损害。Δ bamE突变体的膜具有正常量的外膜蛋白RsaF（TolC同源物），但缺乏CpaC*（IV型皮利的外膜分泌素的聚集形式）。Δ bamE膜还含有大量减少的三种TonB依赖性受体，这些受体在野生型细胞中丰富。缺乏BamE的细胞具有短柄，并且在细胞周期期间柄生长延迟。基于这些发现，我们提出柄杆菌BamE参与外膜β-桶蛋白的组装，包括启动茎生物发生所需的一种或多种底物。
The outer membrane of Gram-negative bacteria is an essential compartment containing a specific complement of lipids and proteins that constitute a protective, selective permeability barrier. Outer membraneβ-barrel proteins are assembled into the membrane by the essential hetero-oligomeric BAM complex, which contains the lipoprotein BamE. We have identified a homologue of BamE, encoded byCC1365, which is located in the outer membrane of the stalked alpha-proteobacteriumCaulobacter crescentus. BamE associates with proteins whose homologues in other bacteria are known to participate in outer membrane protein assembly: BamA (CC1915), BamB (CC1653) and BamD (CC1984).Caulobactercells lacking BamE grow slowly in rich medium and are hypersensitive to anionic detergents, some antibiotics and heat exposure, which suggest that the membrane integrity of the mutant is compromised. Membranes of the ΔbamEmutant have normal amounts of the outer membrane protein RsaF, a TolC homologue, but are deficient in CpaC*, an aggregated form of the outer membrane secretin for type IV pili. ΔbamEmembranes also contain greatly reduced amounts of three TonB-dependent receptors that are abundant in wild-type cells. Cells lacking BamE have short stalks and are delayed in stalk outgrowth during the cell cycle. Based on these findings, we propose thatCaulobacterBamE participates in the assembly of outer membraneβ-barrel proteins, including one or more substrates required for the initiation of stalk biogenesis.
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DOI：
--
发表时间：
2008
期刊：
Proteins: Structure, Function, and Bioinformatics
影响因子：
--
作者：
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DOI：
10.1016/0005-2736(82)90108-0
发表时间：
1982
期刊：
Biochimica et biophysica acta
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
2.8
作者：
Fardini, Yann;Trotereau, Jerome;Virlogeux-Payant, Isabelle
通讯作者：
Virlogeux-Payant, Isabelle
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影响因子：
3.2
作者：
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