Molecular diagnosis of anti-laminin 332 (epiligrin) mucous membrane pemphigoid.
Molecular diagnosis of anti-laminin 332 (epiligrin) mucous membrane pemphigoid.
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DOI：
10.1186/s13023-018-0855-x
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发表时间：
2018-07-06
影响因子：
3.7
通讯作者：
Sitaru C
中科院分区：
文献类型：
--
作者：
Chiorean R;Danescu S;Virtic O;Mustafa MB;Baican A;Lischka A;Hashimoto T;Kariya Y;Koch M;Sitaru C
黏膜类天疱疮是一组以黏膜为主要受累部位的慢性上皮下自身免疫性水疱性疾病。层粘连蛋白332特异性自身抗体存在于约1/3的患者中，与恶性肿瘤风险增加相关。由于严重的并发症，早期识别疾病，及时治疗是必不可少的。用于检测层粘连蛋白332特异性自身抗体的金标准方法，包括免疫沉淀和免疫印迹是非定量的，费力的，并且仅限于全世界的少数专业实验室。此外，放射免疫测定法的使用虽然高度敏感和特异，但费力、昂贵且受到严格管制。因此，迫切需要用于层粘连蛋白332特异性自身抗体的常规检测的定量免疫测定，其更广泛地可用于诊断实验室。本研究的目的是比较不同的抗原底物，包括天然的，重组层粘连蛋白332和层粘连蛋白332丰富的角质形成细胞细胞外基质，ELISA检测粘膜类天疱疮自身抗体的发展。使用相对大量的血清MMP患者的特点良好的自身抗体反应性，我们显示了ELISA系统的适用性，使用层粘连蛋白332制剂作为辅助诊断工具MMP。虽然层粘连蛋白332的糖基化似乎不影响其通过MMP自身抗体的识别，但使用纯化的、天然的和重组的层粘连蛋白332的ELISA系统表现出高灵敏度和与通过免疫印迹检测自身抗体的良好相关性。使用不同层粘连蛋白332制剂的ELISA系统代表了广泛实验室的可行且更容易获得的替代方案。我们的研究结果使层粘连蛋白332丰富的制剂作为粘膜类天疱疮的辅助诊断工具的免疫测定的使用合格。本文的在线版本（10.1186/s13023-018-0855-x）包含补充材料，可供授权用户使用。
Mucous membrane pemphigoid is a group of chronic subepithelial autoimmune blistering diseases that mainly affect mucous membranes. Laminin 332-specific autoantibodies are present in approximately 1/3 of the patients, being associated with an increased risk of malignancy. Because of the severe complications, an early recognition of the disease allowing a timely therapy is essential. The gold standard methods for detection of laminin 332-specific autoantibodies, including the immunoprecipitation and immunoblotting are non-quantitative, laborious and restricted to a few specialized laboratories worldwide. In addition, the use of radioimmunoassays, although highly sensitive and specific, are laborious, expensive and tightly regulated. Therefore, there is a stringent need for a quantitative immunoassay for the routine detection of laminin 332-specific autoantibodies more broadly available to diagnostic laboratories. The aim of this study was to compare different antigenic substrates, including native, recombinant laminin 332 and laminin 332-rich keratinocyte extracellular matrix, for development of an ELISA to detect autoantibodies in mucous membrane pemphigoid. Using a relatively large number of sera from MMP patients with well-characterized autoantibody reactivity we show the suitability of ELISA systems using laminin 332 preparations as adjunct diagnostic tools in MMP. While glycosylation of laminin 332 does not appear to influence its recognition by MMP autoantibodies, ELISA systems using both purified, native and recombinant laminin 332 demonstrated a high sensitivity and good correlation with the detection of autoantibodies by immunoblotting. ELISA systems using different laminin 332 preparations represent a feasible and more accessible alternative for a broad range of laboratories. Our findings qualify the use of immunoassays with the laminin 332-rich preparations as an ancillary diagnostic tool in mucous membrane pemphigoid. The online version of this article (10.1186/s13023-018-0855-x) contains supplementary material, which is available to authorized users.
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影响因子：
3
作者：
Licarete E;Ganz S;Recknagel MJ;Di Zenzo G;Hashimoto T;Hertl M;Zambruno G;Hundorfean G;Mudter J;Neurath MF;Bruckner-Tuderman L;Sitaru C
通讯作者：
Sitaru C
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通讯作者：
YANCEY, KB
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6.5
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Hsu, R;Lazarova, Z;Yancey, KB
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Yancey, KB