Analysis of Pvama1 genes from China-Myanmar border reveals little regional genetic differentiation of Plasmodium vivax populations.
Analysis of Pvama1 genes from China-Myanmar border reveals little regional genetic differentiation of Plasmodium vivax populations.
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中缅边境Pvama1基因分析显示间日疟原虫种群区域遗传分化较小
DOI：
10.1186/s13071-016-1899-1
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发表时间：
2016-11-29
影响因子：
3.2
通讯作者：
Cui L
中科院分区：
文献类型：
--
作者：
Zhu X;Zhao P;Wang S;Liu F;Liu J;Wang J;Yang Z;Yan G;Fan Q;Cao Y;Cui L
背景：在疟疾发病率降低后寄生虫遗传多样性减少的前提下，多态性抗原标记的分析可以为疟疾控制对当地寄生虫种群影响提供重要信息。本研究评估了中缅边境疟原虫顶膜抗原1（Pvama1）基因的遗传多样性，并与全球间日疟原虫种群进行了比较。方法：我们对从中缅边境获得的73个Pvama1序列以及来自全球7个间日疟原虫的615个公开的Pvama1序列进行进化分析，以考察其遗传多样性、自然选择和群体分化。结果：在全球间日疟原虫分离株中共鉴定出 308 个 Pvama1 单倍型。 73个中缅边境寄生虫分离物中Pvama1基因的总体核苷酸多样性为0.008，鉴定出41个单倍型（Hd = 0.958）。结构域 I (DI) 包含大多数 (26/33) 多态性位点。 McDonald Kreitman 测试显示 Pvama1 的胞外域和 DI 存在显着的正选择。中缅边境、泰国（0.01）和缅甸（0.10）之间的注视指数（FST）估计仅显示出轻微的地理遗传差异。值得注意的是，在任何分析的全球分离株中均未检测到 Sal-I 单倍型，而贝伦株仅限于泰国人群。检测到的突变位于预测的 B 细胞表位重叠区域和本质上非结构化/无序区域的外部。结论：本研究揭示了来自中缅边境的间日疟原虫种群中 Pvama1 的高水平遗传多样性，其中 DI 显示出比其他域更强的多样化选择。大湄公河次区域的寄生虫种群的种群细分水平较低。
Background:With the premise of diminishing parasite genetic diversity following the reduction of malaria incidence, the analysis of polymorphic antigenic markers may provide important information about the impact of malaria control on local parasite populations. Here we evaluated the genetic diversity of Plasmodium vivax apical membrane antigen 1 (Pvama1) gene in a parasite population from the China-Myanmar border and compared it with global P. vivax populations.Methods:We performed evolutionary analysis to examine the genetic diversity, natural selection, and population differentiation of 73 Pvama1 sequences acquired from the China-Myanmar border as well as 615 publically available Pvama1 sequences from seven global P. vivax populations.Results:A total of 308 Pvama1 haplotypes were identified among the global P. vivax isolates. The overall nucleotide diversity of Pvama1 gene among the 73 China-Myanmar border parasite isolates was 0.008 with 41 haplotypes being identified (Hd = 0.958). Domain I (DI) harbored the majority (26/33) of the polymorphic sites. The McDonald Kreitman test showed a significant positive selection across the ectodomain and the DI of Pvama1. The fixation index (FST) estimation between the China-Myanmar border, Thailand (0.01) and Myanmar (0.10) showed only slight geographical genetic differentiation. Notably, the Sal-I haplotype was not detected in any of the analyzed global isolates, whereas the Belem strain was restricted to the Thai population. The detected mutations are mapped outside the overlapped region of the predicted B-cell epitopes and intrinsically unstructured/disordered regions.Conclusions:This study revealed high levels of genetic diversity of Pvama1 in the P. vivax parasite population from the China-Myanmar border with DI displaying stronger diversifying selection than other domains. There were low levels of population subdivision among parasite populations from the Greater Mekong Subregion.
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影响因子：
5.4
作者：
HILL W G;ROBERTSON A
通讯作者：
ROBERTSON A
影响因子：
10.7
作者：
Gunasekera, Anusha M.;Wickramarachchi, Thilan;Wirth, Dyann F.
通讯作者：
Wirth, Dyann F.
DOI：
10.1073/pnas.0701464104
发表时间：
2007-07-24
影响因子：
11.1
作者：
Dutta, Sheetij;Lee, Seung Yeon;Lanar, David E.
通讯作者：
Lanar, David E.
影响因子：
5.5
作者：
Dias, Sajani;Somarathna, Maheshika;Udagama, Preethi V.
通讯作者：
Udagama, Preethi V.
影响因子：
2.7
作者：
Cui, Liwang;Yan, Guiyun;Sattabongkot, Jetsumon;Cao, Yaming;Chen, Bin;Chen, Xiaoguang;Fan, Qi;Fang, Qiang;Jongwutiwes, Somchai;Parker, Daniel;Sirichaisinthop, Jeeraphat;Kyaw, Myat Phone;Su, Xin-zhuan;Yang, Henglin;Yang, Zhaoqing;Wang, Baomin;Xu, Jianwei;Zheng, Bin;Zhong, Daibin;Zhou, Guofa
通讯作者：
Zhou, Guofa