Preparation of enzymatically cross-linked sulfated chitosan hydrogel and its potential application in thick tissue engineering
Preparation of enzymatically cross-linked sulfated chitosan hydrogel and its potential application in thick tissue engineering
复制标题
酶交联硫酸化壳聚糖水凝胶的制备及其在厚组织工程中的潜在应用
DOI：
10.1007/s11426-013-4887-8
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发表时间：
2013-05
影响因子：
9.6
通讯作者：
Yuan Zhi
中科院分区：
文献类型：
--
作者：
Wang Wei;Guo Lei;Yu YanYan;Yuan Zhi
为了满足组织工程支架初步血管化的要求，厚膜组织工程支架材料不仅要具有良好的细胞亲和性，而且要具有良好的抗凝血能力。本文以硫酸化壳聚糖（SCTS）为基质，制备了一种酶促交联水凝胶支架。首先合成了硫酸化壳聚糖-羟基苯基丙酸（SCTS-HPA）偶联物，并通过红外光谱和核磁共振氢谱对其结构进行了表征。然后在辣根过氧化物酶（HRP）和过氧化氢（H2 O2）存在下制备酶交联水凝胶。体外评价该水凝胶的凝胶化时间、力学性能、形态学及对人脐静脉内皮细胞（HUVECs）的细胞毒性，体内研究SCTS支架的组织相容性。结果表明，HRP和H2 O2的浓度可以控制脱水水凝胶的凝胶化时间、力学性能和形貌。细胞毒性试验表明，水凝胶浸提液对HUVECs无细胞毒性。体内实验表明，SCTS-HPA支架具有良好的组织相容性，无血栓形成。这些结果表明，SCTS-HPA支架可以作为厚的组织工程支架。
For the requirement of preliminary vascularization, the scaffolds for thick tissue engineering should possess not only good cell affinity, but also anticoagulant ability. In this paper, an enzymatically crosslinked hydrogel scaffold based on sulfated chitosan (SCTS) was prepared. Firstly, sulfated chitosan-hydroxyphenylpionic acid (SCTS-HPA) conjugate was synthesized, and its structure was identified by FITR and1H NMR. And then an enzymatically crosslinked hydrogel was prepared in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). The gelation time, mechanical property, morphology and cytotoxicity to human umbilical vein endothelial cells (HUVECs) of the hydrogel was evaluatedin vitro, the tissue compatibility of SCTS scaffold was studiedin vivo. The results showed that the gelation time, mechanical property, morphology of the dehydrated hydrogel could be controlled by the HRP and H2O2concentration. The cytotoxicity test showed that the hydrogel extracts had no cytotoxicity to HUVECs. Thein vivoassay indicated that SCTS-HPA scaffold showed good tissue compatibility, and no thrombus formation. All these results indicated that the SCTS-HPA scaffold could be used as thick tissue engineering scaffold.
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DOI：
10.1016/1359-0197(90)90173-f
发表时间：
1990
期刊：
International Journal of Radiation Applications and Instrumentation. Part C. Radiation Physics and Chemistry
影响因子：
--
作者：
J. Rosiak;J. Olejniczak;W. Pȩkala
通讯作者：
J. Rosiak;J. Olejniczak;W. Pȩkala
DOI：
--
发表时间：
1981-10
期刊：
No to shinkei = Brain and nerve
影响因子：
--
作者：
K. Hirakawa;K. Hashizume;T. Hayashi
通讯作者：
K. Hirakawa;K. Hashizume;T. Hayashi
DOI：
--
发表时间：
2008
期刊：
Journal of materials science & engineering
影响因子：
--
作者：
Dong Sheng-xiong
通讯作者：
Dong Sheng-xiong
影响因子：
2.5
作者：
M. Terbojevich;C. Carraro;A. Cosani;B. Focher;A. Naggi;G. Torri
通讯作者：
M. Terbojevich;C. Carraro;A. Cosani;B. Focher;A. Naggi;G. Torri
影响因子：
2.3
作者：
L. S. Sefcik;C. E. Petrie Aronin;E. Botchwey
通讯作者：
L. S. Sefcik;C. E. Petrie Aronin;E. Botchwey