Transcription factor CitERF71 activates the terpene synthase gene CitTPS16 involved in the synthesis of E-geraniol in sweet orange fruit.
Transcription factor CitERF71 activates the terpene synthase gene CitTPS16 involved in the synthesis of E-geraniol in sweet orange fruit.
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转录因子CitERF71激活萜烯合酶基因CitTPS16参与甜橙果实中E-香叶醇的合成
DOI：
10.1093/jxb/erx316
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发表时间：
2017-10-13
影响因子：
6.9
通讯作者：
Hancock R
中科院分区：
文献类型：
--
作者：
Li X;Xu Y;Shen S;Yin X;Klee H;Zhang B;Chen K;Hancock R
甜橙果实挥发性E-香叶醇的合成是由CitTPS16催化的，CitTPS16基因在成熟过程中受转录调控。柑橘果实独特的风味取决于可溶性糖、有机酸和挥发性化合物的复杂组合。单萜E-香叶醇是甜橙中一种重要的挥发性物质，对甜橙的风味有重要贡献。此外，还观察到E-香叶醇的抗真菌活性。然而，负责其合成的萜烯合成酶(TPS)尚未在甜橙中被鉴定出来。萜烯合成酶16(CitTPS16)在体外催化E-香叶醇的合成，CitTPS16在纽荷尔甜橙果实和叶片中的瞬时过表达导致了E-香叶醇在体内的积累。在确定了相关的酶之后，我们接下来研究了CitTPS16在水果中的转录调控。在AP2/ERF转录因子基因家族的克隆成员中，CitERF71的表达模式与CitTPS16相似。此外，根据瞬时双荧光素酶试验和酵母单杂交试验的结果，CitERF71能够激活CitTPS16启动子。EMSA分析表明，CitERF71与CitTPS16启动子中的ACCCGCC和GGCGGG基序直接结合。这些结果表明CitERF71在CitTP16的转录调控中发挥重要作用，从而控制柑橘果实中E-香叶醇的产生。
Synthesis of the sweet orange fruit volatile E-geraniol is catalyzed by CitTPS16, and the CitTPS16 gene is transcriptionally regulated during ripening The unique flavor of Citrus fruit depends on complex combinations of soluble sugars, organic acids, and volatile compounds. The monoterpene E-geraniol is an important volatile, contributing to flavor in sweet orange (Citrus sinensis Osbeck). Moreover, antifungal activity of E-geraniol has also been observed. However, the terpene synthase (TPS) responsible for its synthesis has not been identified in sweet orange. Terpene synthase 16 (CitTPS16) was shown to catalyze synthesis of E-geraniol in vitro, and transient overexpression of CitTPS16 in fruits and leaves of Newhall sweet orange resulted in E-geraniol accumulation in vivo. Having identified the responsible enzyme, we next examined transcriptional regulation of CitTPS16 in the fruit. Among cloned members of the AP2/ERF transcription factor gene family, CitERF71 showed a similar expression pattern to CitTPS16. Moreover, CitERF71 was able to activate the CitTPS16 promoter based on results from transient dual-luciferase assays and yeast one-hybrid assays. EMSAs showed that CitERF71 directly binds to ACCCGCC and GGCGGG motifs in the CitTPS16 promoter. These results indicate an important role for CitERF71 in transcriptional regulation of CitTP16 and, therefore, in controlling production of E-geraniol in Citrus fruit.
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DOI：
10.3390/molecules22040591
发表时间：
2017-04-14
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Mirzaei-Najafgholi H;Tarighi S;Golmohammadi M;Taheri P
通讯作者：
Taheri P
影响因子：
6.1
作者：
Antonelli, A;Fabbri, C;Bazzocchi, R
通讯作者：
Bazzocchi, R
影响因子：
3.1
作者：
Aubourg, S;Lecharny, A;Bohlmann, J
通讯作者：
Bohlmann, J
影响因子：
1.6
作者：
Shoji, Tsubasa;Hashimoto, Takashi
通讯作者：
Hashimoto, Takashi
影响因子：
7.2
作者：
Shoji, Tsubasa;Hashimoto, Takashi
通讯作者：
Hashimoto, Takashi