Assembly and budding of Ebolavirus.
Assembly and budding of Ebolavirus.
复制标题
埃博拉病毒的组装和萌芽。
DOI：
10.1371/journal.ppat.0020099
复制
发表时间：
2006-09
期刊：
影响因子：
6.7
通讯作者：
Kawaoka Y
中科院分区：
文献类型：
--
作者：
Noda T;Ebihara H;Muramoto Y;Fujii K;Takada A;Sagara H;Kim JH;Kida H;Feldmann H;Kawaoka Y
埃博拉病毒是导致高致命性出血热的原因。像所有病毒一样，它必须复制其各种成分并将其组装在细胞中，以复制感染性病毒粒子并使其永久存在。为了产生感染性埃博拉病毒，必须产生一种称为核衣壳（NC）的病毒基因组-蛋白质复合物，并将其转运到细胞表面，掺入病毒体，然后从细胞中释放。为了进一步了解埃博拉病毒的生命周期，我们在哺乳动物细胞中表达了各种病毒蛋白，并对其进行了超微结构和生化检查。核蛋白的单独表达导致螺旋管的形成，其可能充当NC的核心。基质蛋白VP 40被发现是至关重要的运输NC到细胞表面和NC到病毒体，其中核蛋白和基质蛋白VP 40之间的相互作用可能是必不可少的这些过程中的掺入。病毒感染细胞的检查显示，含有NC的病毒粒子主要从细胞表面水平出现，而空病毒粒子主要垂直出芽，这表明水平出芽是埃博拉病毒出芽的主要模式。这些数据为识别和开发潜在的抗病毒剂以对抗这种病毒引起的毁灭性疾病奠定了基础。 埃博拉病毒是一种严重的，大多是致命的人类出血热的病原体。像所有的病毒一样，它必须复制它的各种成分，并将它们组装成后代病毒颗粒。然而，由于埃博拉病毒需要特殊的生物安全控制，对其形态发生的理解进展缓慢，导致目前人类没有有效的治疗方法。为了进一步了解埃博拉病毒的生命周期，作者研究了病毒蛋白质之间的特异性相互作用和埃博拉病毒形态发生的过程。研究表明，核蛋白可能作为核衣壳复合物的核心，对病毒基因组的复制和转录至关重要。核蛋白与基质蛋白VP 40的相互作用被发现是必不可少的运输的NC到细胞表面和NC到病毒粒子的掺入，导致在成熟的病毒颗粒的形成。在我们所知的所有病毒中，含有NC的埃博拉病毒颗粒主要从细胞表面水平出现，而其他病毒则垂直出芽。这些发现为识别和开发潜在的抗病毒剂以对抗这种病毒引起的毁灭性疾病奠定了基础。
Ebolavirus is responsible for highly lethal hemorrhagic fever. Like all viruses, it must reproduce its various components and assemble them in cells in order to reproduce infectious virions and perpetuate itself. To generate infectious Ebolavirus, a viral genome-protein complex called the nucleocapsid (NC) must be produced and transported to the cell surface, incorporated into virions, and then released from cells. To further our understanding of the Ebolavirus life cycle, we expressed the various viral proteins in mammalian cells and examined them ultrastructurally and biochemically. Expression of nucleoprotein alone led to the formation of helical tubes, which likely serve as a core for the NC. The matrix protein VP40 was found to be critical for transport of NCs to the cell surface and for the incorporation of NCs into virions, where interaction between nucleoprotein and the matrix protein VP40 is likely essential for these processes. Examination of virus-infected cells revealed that virions containing NCs mainly emerge horizontally from the cell surface, whereas empty virions mainly bud vertically, suggesting that horizontal budding is the major mode of Ebolavirus budding. These data form a foundation for the identification and development of potential antiviral agents to combat the devastating disease caused by this virus. Ebolavirus is a causative agent of a severe, mostly fatal hemorrhagic fever in humans. Like all viruses, it must reproduce its various components and assemble them to reproduce progeny virus particles. However, because Ebolavirus needs special containment for biosafety, progress in understanding its morphogenesis has been slow, resulting in no effective therapies currently available for humans. To further our understanding of the Ebolavirus life cycle, the authors examined the specific interactions among viral proteins and the processes of Ebolavirus morphogenesis. It is demonstrated that nucleoprotein likely serves as a core for the nucleocapsid (NC) complex, which is critical for replication and transcription of viral genome. The interaction of nucleoprotein with matrix protein VP40 is found to be essential for transport of NCs to the cell surface and for the incorporation of NCs into virions, resulting in the formation of mature virus particles. Unique among all viruses to our knowledge, Ebolavirus particles containing NCs mainly emerge horizontally from the cell surface, whereas the other viruses bud vertically. These findings form a foundation for the identification and development of potential antiviral agents to combat the devastating disease caused by this virus.
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影响因子：
3.1
作者：
Kallstrom, G;Warfield, KL;Aman, MJ
通讯作者：
Aman, MJ
影响因子：
5.4
作者：
Noda, T;Sagara, H;Kawaoka, Y
通讯作者：
Kawaoka, Y
埃博拉病毒 VP40 蛋白中的 PPxY 矩阵与泛素连接酶存在物理和功能上的相互作用： 丝状病毒出芽的意义
DOI：
10.1073/pnas.250277297
发表时间：
2000-12-05
影响因子：
11.1
作者：
Harty, RN;Brown, ME;Hayes, FP
通讯作者：
Hayes, FP
影响因子：
7.8
作者：
ALONSO, FV;COMPANS, RW
通讯作者：
COMPANS, RW
影响因子：
3.7
作者：
Becker, S;Rinne, C;Mühlberger, E
通讯作者：
Mühlberger, E