Comparisons of the composition and biogeographic distribution of the bacterial communities occupying South African thermal springs with those inhabiting deep subsurface fracture water
Comparisons of the composition and biogeographic distribution of the bacterial communities occupying South African thermal springs with those inhabiting deep subsurface fracture water
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南非温泉与深层地下裂缝水中细菌群落的组成和生物地理分布的比较
DOI：
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发表时间：
2014
影响因子：
5.2
通讯作者：
T. Onstott
中科院分区：
文献类型：
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作者：
C. Magnabosco;M. Tekere;M. Lau;B. Linage;O. Kuloyo;Mariana Erasmus;E. Cason;E. van Heerden;G. Borgonie;T. Kieft;J. Olivier;T. Onstott
南非有许多温泉，这些温泉代表着地形驱动的大气水沿着主要断裂带迁移。林波波省温泉的温度(40-70°C)和酸碱度(8-9)与南非矿山在1.0至3.1公里深度内遇到的低盐度裂隙水非常相似。这些温泉的主要阳离子和阴离子组成与深部裂隙水非常相似，只是溶解的无机碳和溶解O2在泉水中通常高于深部裂隙水。这种温泉和供给它们的地下压裂液的原位生物相关性以前从未被评估过。在这项研究中，我们使用16S rRNA基因高变区的高通量测序方法，评估了南非六个温泉和六个地下地点的微生物多样性。在地下和温泉环境中，变形杆菌都被鉴定为优势类群，但在所有样品中只有一个属被鉴定为Rheinheimera。使用Morisita相似性指数作为站点之间配对比较的度量，我们发现温泉群落与地下数据集高度不同。尽管林波波温泉似乎没有为观察地下细菌群落提供一个新的窗口，但我们报告说，所研究的地下遗址的分类组成比以前的结果更相似，并提供了证据，表明在深处采样的微生物群落更多地与地下条件有关，而不是地理距离。
South Africa has numerous thermal springs that represent topographically driven meteoric water migrating along major fracture zones. The temperature (40–70°C) and pH (8–9) of the thermal springs in the Limpopo Province are very similar to those of the low salinity fracture water encountered in the South African mines at depths ranging from 1.0 to 3.1 km. The major cation and anion composition of these thermal springs are very similar to that of the deep fracture water with the exception of the dissolved inorganic carbon and dissolved O2, both of which are typically higher in the springs than in the deep fracture water. The in situ biological relatedness of such thermal springs and the subsurface fracture fluids that feed them has not previously been evaluated. In this study, we evaluated the microbial diversity of six thermal spring and six subsurface sites in South Africa using high-throughput sequencing of 16S rRNA gene hypervariable regions. Proteobacteria were identified as the dominant phylum within both subsurface and thermal spring environments, but only one genera, Rheinheimera, was identified among all samples. Using Morisita similarity indices as a metric for pairwise comparisons between sites, we found that the communities of thermal springs are highly distinct from subsurface datasets. Although the Limpopo thermal springs do not appear to provide a new window for viewing subsurface bacterial communities, we report that the taxonomic compositions of the subsurface sites studied are more similar than previous results would indicate and provide evidence that the microbial communities sampled at depth are more correlated to subsurface conditions than geographical distance.
影响因子：
9.3
作者：
Petrosino JF;Highlander S;Luna RA;Gibbs RA;Versalovic J
通讯作者：
Versalovic J