Involvement of the 5'-leader sequence in coupling the stability of a human H3 histone mRNA with DNA replication.
Involvement of the 5'-leader sequence in coupling the stability of a human H3 histone mRNA with DNA replication.
复制标题
5-前导序列参与人 H3 组蛋白 mRNA 稳定性与 DNA 复制的耦合。
DOI：
10.1073/pnas.83.4.981
复制
发表时间：
1986
影响因子：
11.1
通讯作者：
Stein,G
中科院分区：
文献类型：
--
作者：
Morris,T;Marashi,F;Weber,L;Hickey,E;Greenspan,D;Bonner,J;Stein,J;Stein,G
来自融合基因构建体的两条证据表明，细胞周期依赖性人H3组蛋白mRNA 5 '非翻译区中的序列参与了DNA合成终止时发生的选择性不稳定。实验方法是构建嵌合基因，其中H3组蛋白基因的mRNA编码区片段与不以细胞周期依赖性方式表达的基因片段融合。用重组质粒转染HeLa S3细胞后，在DNA合成抑制之前和之后通过S1核酸酶分析测定融合mRNA的水平。当H3组蛋白mRNA前导序列的前20个核苷酸被果蝇热休克（hsp 70）mRNA前导序列的89个核苷酸取代时，融合转录本在DNA合成抑制期间保持稳定，而不是内源性组蛋白mRNA的快速不稳定。在这些细胞中。在一个相互的实验中，构建了一个组蛋白-珠蛋白融合基因，它产生一个转录本，其中H3组蛋白mRNA的前20个核苷酸取代了人β-珠蛋白mRNA的前导序列。在用DNA合成和/或蛋白质合成的抑制剂处理的HeLa细胞中，这种组蛋白-珠蛋白融合mRNA的细胞水平似乎以类似于内源性组蛋白mRNA水平的方式被调节。这些结果表明，前导序列的前20个核苷酸足以将组蛋白mRNA稳定性与DNA复制偶联。
Two lines of evidence derived from fusion gene constructs indicate that sequences residing in the 5'-nontranslated region of a cell cycle-dependent human H3 histone mRNA are involved in the selective destabilization that occurs when DNA synthesis is terminated. The experimental approach was to construct chimeric genes in which fragments of the mRNA coding regions of the H3 histone gene were fused with fragments of genes not expressed in a cell cycle-dependent manner. After transfection in HeLa S3 cells with the recombinant plasmids, levels of fusion mRNAs were determined by S1 nuclease analysis prior to and following DNA synthesis inhibition. When the first 20 nucleotides of an H3 histone mRNA leader were replaced with 89 nucleotides of the leader from a Drosophila heat-shock (hsp70) mRNA, the fusion transcript remained stable during inhibition of DNA synthesis, in contrast to the rapid destabilization of the endogenous histone mRNA in these cells. In a reciprocal experiment, a histone-globin fusion gene was constructed that produced a transcript with the initial 20 nucleotides of the H3 histone mRNA substituted for the human beta-globin mRNA leader. In HeLa cells treated with inhibitors of DNA synthesis and/or protein synthesis, cellular levels of this histone-globin fusion mRNA appeared to be regulated in a manner similar to endogenous histone mRNA levels. These results suggest that the first 20 nucleotides of the leader are sufficient to couple histone mRNA stability with DNA replication.
腺病毒2转化区mRNA的代谢。
DOI：
10.1101/sqb.1980.044.01.048
发表时间：
1980
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Wilson,MC;Nevins,JR;Blanchard,JM;Ginsberg,HS;DarnellJr,JE
通讯作者：
DarnellJr,JE
HeLa细胞的多个H4组蛋白mRNA由不同基因编码
影响因子：
64.8
作者：
A. Lichtler;F. Sierra;S. Clark;J. Wells;J. Stein;G. Stein
通讯作者：
G. Stein