Expression profile of histone deacetylase 1 in gastric cancer tissues.
Expression profile of histone deacetylase 1 in gastric cancer tissues.
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DOI：
10.1111/j.1349-7006.2001.tb02153.x
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发表时间：
2001-12
期刊：
影响因子：
--
通讯作者：
Kim DY
中科院分区：
文献类型：
--
作者：
Choi JH;Kwon HJ;Yoon BI;Kim JH;Han SU;Joo HJ;Kim DY
虽然组蛋白脱乙酰酶（HDAC）似乎在癌症发生中起着至关重要的作用，但HDAC在原发性人类癌症组织中的表达状况尚未报道。在这项研究中，我们通过半定量RT-PCR和免疫印迹分析研究了HDAC 1在25对原发性人胃癌（GC）组织和相应正常组织中的表达水平。还通过免疫组织化学对HDAC 1表达模式进行了拓扑学检查。HDAC 1 mRNA在68%的胃癌组织中过表达（17/25），与正常组织相比，胃癌组织中HDAC 1 mRNA的相对密度增加了1.8倍（P<0.01）。在61%的GC样品（18个样品中的11个）中也检测到HDAC 1蛋白的表达升高，这也表明HDAC的mRNA水平升高。免疫组化结果显示，HDAC 1的过度表达主要位于大多数肿瘤细胞的细胞核中，包括栓塞性肿瘤细胞，而正常腺上皮细胞仅显示微弱的HDAC 1表达，分布集中。因此，本研究清楚地表明，HDAC 1在GC中过表达，并可能在胃癌发生中发挥重要作用。
Although histone deacetylases (HDACs) appear to play a crucial role in carcinogenesis, the expression status of HDACs in primary human cancer tissues has not yet been reported. In this study, we investigated the expression level of HDAC1 in 25 paired primary human gastric cancer (GC) tissues and corresponding normal tissues through semi‐quantitative RT‐PCR and immunoblot analysis. The HDAC1 expression pattern was also topologically examined through immunohisto‐chemistry. Overexpression of HDAC1 mRNA was detected in 68% of GC tissues (17 of 25), and the relative density of HDAC1 mRNA in GC tissue was increased 1.8‐fold versus the normal counterpart (P<0.01). Elevated expression of HDAC1 protein was also detected in 61% of GC samples (11 of 18), which also showed an increased mRNA level of HDAC. Immunohistochemically, overexpression of HDAC1 was predominantly localized in the nuclei of most neoplastic cells, including embolic tumor cells, whereas normal glandular epithelial cells revealed only weak HDAC1 expression that was focal in distribution. Thus, the present study clearly demonstrates that HDAC1 is overexpressed in GC and probably plays a significant role in gastric carcinogenesis.
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DOI：
10.1006/bbrc.1998.8891
发表时间：
1998-06-29
影响因子：
3.1
作者：
Dangond, F;Gullans, SR
通讯作者：
Gullans, SR
影响因子：
64.8
作者：
PETRIJ, F;GILES, RH;BREUNING, MH
通讯作者：
BREUNING, MH
影响因子：
82.9
作者：
Kim, MS;Kwon, HJ;Kim, KW
通讯作者：
Kim, KW
影响因子：
3.5
作者：
Kim, YB;Honda, A;Horinouchi, S
通讯作者：
Horinouchi, S
DOI：
10.1006/bbrc.1998.8449
发表时间：
1998-04-17
影响因子：
3.1
作者：
Gray, SG;Ekström, TJ
通讯作者：
Ekström, TJ