Hypoxia-induced hsa_circ_0000826 is linked to liver metastasis of colorectal cancer.
Hypoxia-induced hsa_circ_0000826 is linked to liver metastasis of colorectal cancer.
复制标题
DOI：
10.1002/jcla.23405
复制
发表时间：
2020-09
影响因子：
2.7
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Shi L;Tao C;Tang Y;Xia Y;Li X;Wang X
hsa_circ_0000826 has been previously linked to CRC through the competing endogenous RNA network; however, the upstream driver of hsa_circ_0000826 elevation remains unknown. In this study, we aim to elucidate the effect of hypoxia‐induced hsa_circ_0000826 on CRC tumorigenesis and metastasis. RNA scope assay was used to evaluate the expression of hsa_circ_0000826 in CRC cells under hypoxia condition. The effects of hsa_circ_0000826 on phenotypes of CRC cells were evaluated through cell migration and invasion assay. The nude, AOM‐DSS model mice and APCMin /+ mice were used to investigate the relationship between circ_0000826, hypoxia, and CRC in mice. A total of 100 CRC tissue samples, as well as the paired adjacent tissues, were collected, and qRT‐PCR assay was used to detect the expression of hsa_circ_0000826 in these samples. Hypoxia‐induced hsa_circ_0000826 overexpression can increase the malignant phenotypes, tumor formation, and metastasis capability of CRC cells in vitro. mmu_circ_0000826 levels were significantly increased in the CRC tissues from AOM‐DSS and APC mice model under hypoxia conditions. Further, the hypoxia‐induced upregulation of mmu_circ_0000826 can also promote CRC tumorigenesis and liver metastasis in vivo. The expression of hsa_circ_0000826 in serum was significantly increased in CRC tissues in 100‐pair of CRC and according to the adjacent normal tissues by qRT‐PCR assays. Moreover, the expression levels of hsa_circ_0000826 in serum of patient with liver metastasis were significantly increased than those without metastasis. Our results suggested that hsa_circ_0000826 was induced by the hypoxia in CRC, which can be a potential biomarker of CRC liver metastasis.
登录
查看更多内容
影响因子：
5.6
作者：
Mosińska P;Gabryelska A;Zasada M;Fichna J
通讯作者：
Fichna J
影响因子：
4.1
作者：
Dudekulay, Dawood B.;Panda, Amaresh C.;Gorospe, Myriam
通讯作者：
Gorospe, Myriam
影响因子：
2.7
作者：
Hanna JA;Wimberly H;Kumar S;Slack F;Agarwal S;Rimm DL
通讯作者：
Rimm DL
影响因子：
4.5
作者：
Salzman J;Chen RE;Olsen MN;Wang PL;Brown PO
通讯作者：
Brown PO
影响因子：
254.7
作者：
Islami, Farhad;Miller, Kimberly D.;Jemal, Ahmedin
通讯作者：
Jemal, Ahmedin