Chromosomal copy number variation analysis by next generation sequencing confirms ploidy stability in Trypanosoma brucei subspecies.
Chromosomal copy number variation analysis by next generation sequencing confirms ploidy stability in Trypanosoma brucei subspecies.
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DOI：
10.1099/mgen.0.000223
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发表时间：
2018-10
影响因子：
3.9
通讯作者：
Reis-Cunha JL
中科院分区：
文献类型：
--
作者：
Almeida LV;Coqueiro-Dos-Santos A;Rodriguez-Luiz GF;McCulloch R;Bartholomeu DC;Reis-Cunha JL
虽然非整倍体通常会导致多细胞真核生物的严重异常，但最近的数据表明，它可能对单细胞真核生物有益，如酵母和锥虫寄生虫，在压力条件下增加存活率。在特征锥虫中，克氏锥虫、布氏锥虫和利什曼原虫属的物种由于其在公共卫生中的重要性而脱颖而出，感染了全世界约2000万人。T.最近通过基于下一代测序（NGS）和荧光原位杂交的分析证实了克氏疟原虫和利什曼原虫的耐药性，其中它们与昆虫载体和哺乳动物宿主之间传播期间的适应以及促进耐药性有关。尽管上述物种中存在染色体拷贝数变异（CCNVs），但PFGE和荧光细胞光度分析表明，T.布鲁塞。对T.基于NGS读数的B gambiense证实了该亚种中不存在非整倍性。然而，在其他两个T。brucei subspecies，T. B. brucei和T. B. Rhodesiense，尚未使用NGS方法进行评估。在本工作中，我们检测了26个T。布氏杆菌分离株，包括来自三个T.通过等位基因频率和读段深度覆盖分析，这些分析表明，T.布氏亚种呈现非整倍性，这可能与这些寄生虫与利什曼原虫相比在DNA复制和重组机制上的差异有关。
Although aneuploidy usually results in severe abnormalities in multicellular eukaryotes, recent data suggest that it could be beneficial for unicellular eukaryotes, such as yeast and trypanosomatid parasites, providing increased survival under stressful conditions. Among characterized trypanosomatids, Trypanosoma cruzi, Trypanosoma brucei and species from the genus Leishmania stand out due to their importance in public health, infecting around 20 million people worldwide. The presence of aneuploidies in T. cruzi and Leishmania was recently confirmed by analysis based on next generation sequencing (NGS) and fluorescence in situ hybridization, where they have been associated with adaptation during transmission between their insect vectors and mammalian hosts and in promoting drug resistance. Although chromosomal copy number variations (CCNVs) are present in the aforementioned species, PFGE and fluorescence cytophotometry analyses suggest that aneuploidies are absent from T. brucei. A re-evaluation of CCNV in T. b gambiense based on NGS reads confirmed the absence of aneuploidies in this subspecies. However, the presence of aneuploidies in the other two T. brucei subspecies, T. b. brucei and T. b. rhodesiense, has not been evaluated using NGS approaches. In the present work, we tested for aneuploidies in 26 T. brucei isolates, including samples from the three T. brucei subspecies, by both allele frequency and read depth coverage analyses. These analyses showed that none of the T. brucei subspecies presents aneuploidies, which could be related to differences in the mechanisms of DNA replication and recombination in these parasites when compared with Leishmania.
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影响因子：
6.4
作者：
Dumetz F;Imamura H;Sanders M;Seblova V;Myskova J;Pescher P;Vanaerschot M;Meehan CJ;Cuypers B;De Muylder G;Späth GF;Bussotti G;Vermeesch JR;Berriman M;Cotton JA;Volf P;Dujardin JC;Domagalska MA
通讯作者：
Domagalska MA
影响因子：
3.2
作者：
Gibson, Wendy;Peacock, Lori;Bailey, Mick
通讯作者：
Bailey, Mick
影响因子：
56.9
作者：
Ivens, AC;Peacock, CS;Myler, PJ
通讯作者：
Myler, PJ
影响因子：
12.3
作者：
Marques, Catarina A.;Dickens, Nicholas J.;McCulloch, Richard
通讯作者：
McCulloch, Richard
影响因子：
1.5
作者：
GIBSON, WC;OSINGA, KA;BORST, P
通讯作者：
BORST, P