RIM-BP3 is a manchette-associated protein essential for spermiogenesis
RIM-BP3 is a manchette-associated protein essential for spermiogenesis
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RIM-BP3 是精子发生必需的 manchette 相关蛋白
DOI：
10.1242/dev.030858
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发表时间：
2009-02
期刊：
影响因子：
4.6
通讯作者：
Ahmed Mansouri
中科院分区：
文献类型：
--
作者：
杜雅蕊;黄燕女;韩代书;胡业广;周静;鲍兰;秦维华;徐国良;Ahmed Mansouri
在精子发生过程中，哺乳动物的圆形精子细胞转化为活动精子。对精子形态发生的机制知之甚少。我们已经表征了一种新的蛋白质，RIM-BP 3，在小鼠精子细胞发育中具有专门的功能。RIM-BP 3蛋白与曼切特相关，曼切特是一种短暂的微管结构，被认为对精子发生过程中的形态发生很重要。RIM-BP 3基因的靶向缺失导致由于异常精子头部的男性不育，其特征在于变形的核和分离的顶体。与其在形态发生中的作用相一致，RIM-BP 3蛋白与Hook 1（一种已知的精子头部形态发生所需的manchette结合蛋白）物理关联。有趣的是，RIM-BP 3不与在azh（异常精子头）突变小鼠中表征的截短的Hook 1蛋白相互作用。此外，RIM-BP 3和Hook 1突变小鼠表现出几种常见的异常，特别是在精子细胞内的异位定位，精子头部畸形的推测原因。这些观察结果表明，RIM-BP 3通过与Hook 1的相互作用在Manchette发育和功能中发挥重要作用。由于畸形精子细胞的发生是导致精子功能障碍的常见异常之一，因此RIM-BP 3的鉴定可能提供对人类男性不育症潜在原因的分子线索的了解。
During spermiogenesis, round spermatids are converted into motile sperm in mammals. The mechanisms responsible for sperm morphogenesis are poorly understood. We have characterized a novel protein, RIM-BP3, with a specialized function in spermatid development in mice. The RIM-BP3 protein is associated with the manchette, a transient microtubular structure believed to be important for morphogenesis during spermiogenesis. Targeted deletion of the RIM-BP3 gene resulted in male infertility owing to abnormal sperm heads, which are characterized by a deformed nucleus and a detached acrosome. Consistent with its role in morphogenesis, the RIM-BP3 protein physically associates with Hook1, a known manchette-bound protein required for sperm head morphogenesis. Interestingly, RIM-BP3 does not interact with the truncated Hook1 protein characterized in azh (abnormal spermatozoon head) mutant mice. Moreover, RIM-BP3 and Hook1 mutant mice display several common abnormalities, in particular with regard to the ectopic positioning of the manchette within the spermatid, a presumed cause of sperm head deformities. These observations suggest an essential role for RIM-BP3 in manchette development and function through its interaction with Hook1. As the occurrence of deformed spermatids is one of the common abnormalities leading to malfunctional sperm, identification of RIM-BP3 might provide insight into the molecular cue underlying causes of male infertility in humans.
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DOI：
10.1073/pnas.162027899
发表时间：
2002-07
影响因子：
11.1
作者：
R. Yao;C. Ito;Yasuko Natsume;Yoshinobu Sugitani;H. Yamanaka;Shoji Kuretake;K. Yanagida;A. Sato;K. Toshimori;T. Noda
通讯作者：
R. Yao;C. Ito;Yasuko Natsume;Yoshinobu Sugitani;H. Yamanaka;Shoji Kuretake;K. Yanagida;A. Sato;K. Toshimori;T. Noda
DOI：
10.1073/pnas.0406060102
发表时间：
2005-02-22
影响因子：
11.1
作者：
Martianov, I;Brancorsini, S;Davidson, I
通讯作者：
Davidson, I
影响因子：
2.4
作者：
Y. Toyama;T. Kazama;H. Fuse;T. Katayama
通讯作者：
Y. Toyama;T. Kazama;H. Fuse;T. Katayama
DOI：
10.1002/ajmg.10658
发表时间：
2002-09-15
期刊：
AMERICAN JOURNAL OF MEDICAL GENETICS
影响因子：
--
作者：
Shirohzu, H;Kubota, T;Sasaki, H
通讯作者：
Sasaki, H
影响因子：
2.5
作者：
Kierszenbaum, AL
通讯作者：
Kierszenbaum, AL