Gene Expression Patterns Associated with in Vitro Tracheary Element Formation in Isolated Single Mesophyll Cells of Zinnia elegans
Gene Expression Patterns Associated with in Vitro Tracheary Element Formation in Isolated Single Mesophyll Cells of Zinnia elegans
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与百日草分离的单个叶肉细胞体外气管元件形成相关的基因表达模式
DOI：
10.1104/pp.103.3.805
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发表时间：
1993
期刊：
影响因子：
7.4
通讯作者：
J. E. Varner
中科院分区：
文献类型：
--
作者：
Zheng;J. E. Varner
分离的百日草叶叶肉细胞形成的气管元件是解剖图案化次生细胞壁增厚和木质化的绝佳系统。我们使用在诱导培养基中培养 48 小时的细胞的 mRNA 来分离差异调节的基因。使用消减杂交方法分离出 13 个独特的 cDNA 克隆。根据这些克隆在不同培养基中的特征基因表达，可以将它们分为三个不同的组。第一组包括那些在不含1-萘乙酸(NAA)和苄基腺嘌呤的基础培养基中诱导表达的基因；这表明这些基因的表达受到这些激素以外的化学和物理因素的调节。其中三个克隆 p48h-229、p48h-114 和 p48h-102 分别与发病机制相关蛋白 II、丝氨酸蛋白酶抑制剂和向日葵花药特异性富含脯氨酸蛋白显示出显着同源性。第二组包括那些主要由NAA诱导表达的基因。这些克隆之一 p48h-10 显示出与大麦糊粉特异性 cDNA B11E 的高蛋白质序列同源性。 p48h-10 编码的蛋白质具有植物非特异性脂质转移蛋白的一些共同特征（低分子量、分泌信号肽、八个保守的半胱氨酸残基和碱性蛋白质），尽管 p48h-10 和其他植物非特异性脂质转移蛋白之间没有发现显着的蛋白质序列同源性。第三组包括那些主要在诱导培养基中诱导表达的基因；这表明这些基因的表达与气管元件的形成过程密切相关。其中两个克隆 p48h-107 和 p48h-17 分别与腺苷酸激酶和木瓜蛋白酶 I 显示出高度同源性。讨论了这些分化特异性基因在气管元件形成过程中的可能作用。
Tracheary element formation from isolated Zinnia leaf mesophyll cells is an excellent system for the dissection of patterned secondary cell wall thickening and lignification. We used mRNAs from cells cultured for 48 h in the induction medium to isolate differentially regulated genes. Thirteen unique cDNA clones were isolated using a subtractive hybridization method. These clones can be divided into three distinct groups according to their characteristic gene expression in different media. The first group includes those genes whose expression is induced in the basal medium without 1-naphthaleneacetic acid (NAA) and benzyladenine; this indicates that the expression of these genes is regulated by chemical and physical factors other than these hormones. Three of these clones, p48h-229, p48h-114, and p48h-102, show significant homology to a pathogenesis-related protein II, a serine proteinase inhibitor, and a sunflower anther-specific proline-rich protein, respectively. The second group includes those genes whose expression is mainly NAA induced. One of these clones, p48h-10, shows high protein sequence homology to a barley aleurone-specific cDNA, B11E. The p48h-10-encoded protein shares some common characteristics of plant nonspecific lipid transfer proteins (low molecular weight, the secretion signal peptide, eight conserved cysteine residues, and a basic protein), although no significant protein sequence homology is found between p48h-10 and other plant nonspecific lipid transfer proteins. The third group includes those genes whose expression is induced primarily in the induction medium; this indicates that the expression of these genes is closely associated with the process of tracheary element formation. Two of these clones, p48h-107 and p48h-17, show high homology to adenylate kinase and papaya proteinase I, respectively. The possible roles of these differentiation-specific genes during tracheary element formation are discussed.
影响因子：
7.4
作者：
Diane L. Church;A. Galston
通讯作者：
Diane L. Church;A. Galston
4-香豆酸：诱导分化为气管元件的百日草叶肉细胞中辅酶 A 连接酶和异过氧化物酶的表达。
影响因子：
7.4
作者：
Church,DL;Galston,AW
通讯作者：
Galston,AW