Killing them with kindness? In-hive medications may inhibit xenobiotic efflux transporters and endanger honey bees.
Killing them with kindness? In-hive medications may inhibit xenobiotic efflux transporters and endanger honey bees.
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DOI：
10.1371/journal.pone.0026796
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Dively GP
中科院分区：
文献类型：
--
作者：
Hawthorne DJ;Dively GP
蜜蜂最近经历了比正常情况下更高的越冬蜂群损失。造成损失的原因有很多，其中包括蜂箱中残留的杀虫剂和兽药。多个残留物同时存在，尽管大多数情况下是低浓度的，因此还没有一个产品与损失有关。然而，可能不排除残留物与损失的组合。为了了解暴露在复合残留物中对蜜蜂的影响，我们提出了一个基于机制的策略，这里重点关注多药耐药(MDR)转运体作为这些相互作用的中介。采用全动物生物测定法，通过维拉帕米的抑制作用，我们证明了广泛使用的有机磷和拟除虫菊酯类杀螨剂敌敌畏和氟氰菊酯，以及三种新烟碱类杀虫剂：吡虫啉、扑热息痛和噻虫啉是一种或多种τ转运蛋白的底物。蜂房内的抗生素土霉素是蜜蜂多药耐药转运体的候选抑制剂之一。摄食土霉素的蜜蜂对杀螨剂敌敌畏和τ-氟氰菊酯有显著的增敏作用，提示该抗生素可能干扰了这些农药的正常排泄或代谢。许多蜂箱定期接受土霉素和杀螨剂的治疗，以预防和治疗疾病和寄生虫。我们的结果表明，季节性地在蜂箱中联合使用这些药物可能会增加这些杀虫剂以及其他杀虫剂的不良影响。我们的结果也证明了基于机制的策略的实用性。通过确定作为异种转运蛋白底物和抑制剂的杀虫剂和养蜂药物，我们优先测试那些最可能导致不良相互作用的化学组合。
Honey bees (Apis mellifera) have recently experienced higher than normal overwintering colony losses. Many factors have been evoked to explain the losses, among which are the presence of residues of pesticides and veterinary products in hives. Multiple residues are present at the same time, though most often in low concentrations so that no single product has yet been associated with losses. Involvement of a combination of residues to losses may however not be excluded. To understand the impact of an exposure to combined residues on honey bees, we propose a mechanism-based strategy, focusing here on Multi-Drug Resistance (MDR) transporters as mediators of those interactions. Using whole-animal bioassays, we demonstrate through inhibition by verapamil that the widely used organophosphate and pyrethroid acaricides coumaphos and τ-fluvalinate, and three neonicotinoid insecticides: imidacloprid, acetamiprid and thiacloprid are substrates of one or more MDR transporters. Among the candidate inhibitors of honey bee MDR transporters is the in-hive antibiotic oxytetracycline. Bees prefed oxytetracycline were significantly sensitized to the acaricides coumaphos and τ-fluvalinate, suggesting that the antibiotic may interfere with the normal excretion or metabolism of these pesticides. Many bee hives receive regular treatments of oxytetracycline and acaricides for prevention and treatment of disease and parasites. Our results suggest that seasonal co-application of these medicines to bee hives could increase the adverse effects of these and perhaps other pesticides. Our results also demonstrate the utility of a mechanism-based strategy. By identifying pesticides and apicultural medicines that are substrates and inhibitors of xenobiotic transporters we prioritize the testing of those chemical combinations most likely to result in adverse interactions.
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DOI：
10.1523/jneurosci.5564-08.2009
发表时间：
2009-03-18
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Mayer F;Mayer N;Chinn L;Pinsonneault RL;Kroetz D;Bainton RJ
通讯作者：
Bainton RJ
影响因子：
3.5
作者：
Lanning, CL;Ayad, HM;AbouDonia, MB
通讯作者：
AbouDonia, MB
影响因子：
6.1
作者：
Laurent, FM;Rathahao, E
通讯作者：
Rathahao, E
DOI：
10.1016/s0305-0491(98)10137-2
发表时间：
1998-12-01
影响因子：
2.2
作者：
Podsiadlowski, L;Matha, V;Vilcinskas, A
通讯作者：
Vilcinskas, A
影响因子：
1.9
作者：
Porretta, D.;Gargani, M.;Urbanelli, S.
通讯作者：
Urbanelli, S.