Comparison of bacterial communities in sands and water at beaches with bacterial water quality violations.
Comparison of bacterial communities in sands and water at beaches with bacterial water quality violations.
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DOI：
10.1371/journal.pone.0090815
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Gast RJ
中科院分区：
文献类型：
--
作者：
Halliday E;McLellan SL;Amaral-Zettler LA;Sogin ML;Gast RJ
可培养的粪便指示细菌（FIB）测量的回灌水质，可能会受到这些细菌在当地的沙子或残骸，除了从人类和/或动物来源的不同粪便输入持久的人口的影响。在这项研究中，焦磷酸测序被用来产生短序列标签的16 S高变区核糖体DNA从浅水样品和沙子样品收集在高潮线和在潮间带的水线在网站有和没有FIB免疫事件。这些数据被用来检查沙子和水的细菌群落，以评估样品之间的相似性，并确定水质污染事件对群落组成的影响。属于一组细菌先前确定为替代粪便指标的序列也进行了分析，水质违规事件的关系。我们发现，沙子和水样本托管明显不同的整体细菌群落，并有更大的相似性，从两个遥远的网站之间的沿海水样的社区组成。高潮和潮间带砂细菌群落之间的差异，虽然更类似于彼此比水，对应于更大的潮汐范围之间的样品。在组内的替代粪便指标更大的相似性内观察到的沙子和水从同一网站，可能反映在每个海滩的人为贡献。这项研究支持越来越多的证据表明，基于社区的分子工具可以用来识别环境中粪便污染的来源和潜在影响，并进一步表明，海滩沙子和水中更多样化的细菌群落可能反映出污染较少的场地和更好的水质。
Recreational water quality, as measured by culturable fecal indicator bacteria (FIB), may be influenced by persistent populations of these bacteria in local sands or wrack, in addition to varied fecal inputs from human and/or animal sources. In this study, pyrosequencing was used to generate short sequence tags of the 16S hypervariable region ribosomal DNA from shallow water samples and from sand samples collected at the high tide line and at the intertidal water line at sites with and without FIB exceedance events. These data were used to examine the sand and water bacterial communities to assess the similarity between samples, and to determine the impact of water quality exceedance events on the community composition. Sequences belonging to a group of bacteria previously identified as alternative fecal indicators were also analyzed in relationship to water quality violation events. We found that sand and water samples hosted distinctly different overall bacterial communities, and there was greater similarity in the community composition between coastal water samples from two distant sites. The dissimilarity between high tide and intertidal sand bacterial communities, although more similar to each other than to water, corresponded to greater tidal range between the samples. Within the group of alternative fecal indicators greater similarity was observed within sand and water from the same site, likely reflecting the anthropogenic contribution at each beach. This study supports the growing evidence that community-based molecular tools can be leveraged to identify the sources and potential impact of fecal pollution in the environment, and furthermore suggests that a more diverse bacterial community in beach sand and water may reflect a less contaminated site and better water quality.
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影响因子：
2.4
作者：
Hartz, A.;Cuvelier, M.;Rogerson, A.
通讯作者：
Rogerson, A.
DOI：
10.4319/lom.2010.8.146
发表时间：
2010-04
期刊：
Limnology and oceanography, methods
影响因子：
--
作者：
Halliday E;Griffith JF;Gast RJ
通讯作者：
Gast RJ
影响因子：
5.8
作者：
Bonilla, Tonya D.;Nowosielski, Kara;Rogerson, Andrew
通讯作者：
Rogerson, Andrew
影响因子：
3.6
作者：
Hewson, I;Fuhrman, JA
通讯作者：
Fuhrman, JA
影响因子：
4.2
作者：
Feng, Fan;Goto, Dustin;Yan, Tao
通讯作者：
Yan, Tao