Alternative splicing caused by RNA secondary structure

Alternative splicing caused by RNA secondary structure
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RNA二级结构引起的选择性剪接

DOI:
10.1016/0092-8674(85)90239-9
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发表时间:
1985
期刊:
影响因子:
64.5
通讯作者:
D. Solnick
D. Solnick
中科院分区:
生物学1区
文献类型:
--
作者:
D. Solnick

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Summary mRNA precursors with stable hairpins were constructed by inserting inverted repeats into an adenovirus transcriptional template that encoded the three late leader exons. When the loop of the hairpin contained the second exon and the flanking splice sites, most of the RNA spliced in vitro had the first exon joined directly to the third exon. The remainder was spliced normally. The same types of alternatively spliced RNAs were formed when a similar template was introduced into HeLa cells by transfection. Thus both in extracts and in cells, an exon became optional when sequestered in a hairpin loop. Perhaps a related mechanism creates the alternative splicing patterns of complex transcription units.
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