DNA sequence and product analysis of the virF locus responsible for congo red binding and cell invasion in Shigella flexneri 2a
DNA sequence and product analysis of the virF locus responsible for congo red binding and cell invasion in Shigella flexneri 2a
复制标题
福氏志贺氏菌 2a 中负责刚果红结合和细胞侵袭的 virF 位点的 DNA 序列和产物分析
作者：
T. Sakai;C. Sasakawa;S. Makino;M. Yoshikawa
测定并分析了福氏志贺氏菌2a中与毒力和结合刚果红能力相关的位点virF的DNA序列，该位点位于140兆道尔（230千碱基）质粒上。用氯霉素耐药试剂盒测定了virF的转录方向。找到一个在这个方向上可读的开放阅读框。在含有virF位点的微型细胞中产生了三种蛋白质，分别为30,27,21千道尔顿，均与上述序列预测的蛋白质相对应。这三种蛋白在含有23万碱基质粒的微型细胞中表达较弱。
The DNA sequence of virF, a locus associated with virulence and the ability to bind Congo red in Shigella flexneri 2a that is located on a 140-megadalton (230-kilobase) plasmid, was determined and analyzed. It was rich in A and T. The direction of transcription of virF was determined by using a chloramphenicol resistance cartridge. An open reading frame readable in this direction was found. Three proteins, 30, 27, and 21 kilodaltons, all corresponding to those predicted from the above sequence, were produced in minicells containing the virF locus. The three proteins were expressed only weakly in minicells with the 230-kilobase plasmid.
编码 Eco RI 核酸内切酶和甲基化酶的 DNA 的序列分析。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Greene,PJ;Gupta,M;Boyer,HW;Brown,WE;Rosenberg,JM
通讯作者：
Rosenberg,JM
IS50 介导的逆转座。
影响因子：
5.6
作者：
Sasakawa,C;Berg,DE
通讯作者：
Berg,DE