Interference of hemolysis on the postmortem biochemical analysis of IgE by ECLIA.
Interference of hemolysis on the postmortem biochemical analysis of IgE by ECLIA.
复制标题
DOI：
10.1007/s00414-021-02578-z
复制
发表时间：
2021-07
影响因子：
2.1
通讯作者：
Cao Z
中科院分区：
文献类型：
--
作者：
Wang T;Li Z;Jia Y;Zhu B;Cao Z
过敏性休克的法医学诊断是一个具有挑战性的任务，在法医学实践中，由于缺乏特征性的形态学变化。尸检血清IgE分析可为判断过敏反应提供有用的信息。然而，死后血清总是遭受溶血。为探讨溶血对电致发光免疫分析法（ECLIA）测定总IgE的干扰，验证市售ECLIA试剂盒稀释校正法测定溶血血清的适用性。然后对完全溶血血液和相应血清中的总IgE浓度进行线性回归分析。我们的结果表明，溶血对ECLIA分析总IgE产生负干扰，|偏倚%|）随着溶血水平的增加而增加。在控制|偏倚%|经稀释后，完全溶血血中总IgE的检测浓度仍显著低于血清中总IgE的检测浓度（P < 0.05），出现8例假阴性。完全溶血血液中总IgE的检测浓度与血清中总IgE的检测浓度之间存在较强的相关性（r = 0.983）。经回归公式校正后，与血清总IgE浓度相比，校正后的浓度无显著差异，并显示出相同的诊断能力。结果表明，不建议直接用完全溶血的血液进行总IgE的死后分析。稀释校正法可能具有潜在的实用价值，在法医实践中评估血清总IgE浓度。
Forensic diagnosis of anaphylactic shock is a challenging task in forensic practice due to the lack of characteristic morphological changes. Postmortem analysis of serum IgE can provide helpful information for determining anaphylaxis. However, postmortem serum always suffers from hemolysis. To investigate the interference of hemolysis on postmortem analysis of total IgE by electrochemiluminescent immunoassay (ECLIA) and verify the suitability of the commercially available ECLIA kit for postmortem hemolyzed blood with the dilution-correction method, different levels of hemolyzed serum were prepared to evaluate the interference of hemolysis. A linear regression analysis was then performed on the concentration of total IgE in the completely hemolyzed blood and the corresponding serum. Our results indicated that hemolysis negatively interfered with the total IgE analysis by ECLIA and the interference (|Bias%|) increased with increasing levels of hemolysis. After controlling for |Bias%| by dilution, the test concentration of total IgE in the completely hemolyzed blood was still significantly lower than that in the serum (P < 0.05) and resulted in eight false-negative cases. A strong correlation was observed between the test concentration of total IgE in the completely hemolyzed blood and that in the serum (r = 0.983). After correction by the regression formula, the corrected concentration revealed no significant differences and exhibited the same diagnostic ability, compared with the serum total IgE concentration. These results indicate that the completely hemolyzed blood is not recommended for postmortem analysis of total IgE directly. The dilution-correction method might have potential utility in forensic practice for evaluating serum total IgE concentrations.
登录
查看更多内容
影响因子：
2.2
作者：
Syme, Neil R.;Toft, Anthony D.;Beckett, Geoff J.
通讯作者：
Beckett, Geoff J.
影响因子：
3.5
作者：
ANSARI, MQ;ZAMORA, JL;LIPSCOMB, MF
通讯作者：
LIPSCOMB, MF
影响因子：
2.1
作者：
Nishio, H;Takai, S;Suzuki, K
通讯作者：
Suzuki, K
DOI：
10.1007/s10096-018-3266-y
发表时间：
2018-08-01
影响因子：
4.5
作者：
Teimouri, Aref;Modarressi, Mohammad Hossein;Keshavarz, Hossein
通讯作者：
Keshavarz, Hossein
影响因子：
1.6
作者：
Jia, Yuqing;Tian, Meihui;Cao, Zhipeng
通讯作者：
Cao, Zhipeng