Chromosome 6 encoded RNaseT2 protein is a cell growth regulator.
Chromosome 6 encoded RNaseT2 protein is a cell growth regulator.
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DOI：
10.1111/j.1582-4934.2009.00749.x
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发表时间：
2010-05
影响因子：
5.3
通讯作者：
Athwal RS
中科院分区：
文献类型：
--
作者：
Liu J;Zhawar VK;Kaur G;Kaur GP;Deriel JK;Kandpal RP;Athwal RS
我们先前已经通过染色体转移技术表明，6号染色体改变了多种肿瘤细胞和SV 40永生化细胞的表型。我们在这里提出的表型效应的RNA酶T2，高度保守的核糖核酸酶编码的染色体6 q27，在SV 40永生化细胞系的异位表达。我们将我们的研究结果与卵巢癌细胞系和RNA酶T2转染的SV 40永生化细胞系的研究结果进行了对比。尽管RNaseT 2表达在接近衰老的正常二倍体成纤维细胞中升高（第64代），但在永生化细胞中基因的强制表达不会导致它们衰老。观察到转染RNaseT 2的细胞的集落形成效率、锚定独立性和生长速率显著降低。RNaseT 2表达后，SV 40永生化细胞中Akt信号通路、细胞周期调控和细胞增殖相关通路的转录水平降低2-10倍。有趣的是，一些永生化细胞表达选择性剪接的转录变体，而不是全长RNaseT 2转录。我们的结果与RNaseT 2是细胞生长调节剂并且它不诱导SV 40永生化细胞系衰老的结论一致。
We have previously shown by chromosome transfer technique that chromosome 6 alters the phenotype of a variety of tumour cells and SV40 immortalized cells. We present here the phenotypic effects of the ectopic expression of RNaseT2, a highly conserved ribonuclease encoded by chromosome 6q27, in SV40 immortalized cell lines. We contrast our findings with those reported for ovarian carcinoma cell lines and an SV40 immortalized cell line transfected with RNaseT2. Although RNaseT2 expression is elevated in normal diploid fibroblasts approaching senescence (passage 64), forced expression of the gene in immortalized cells does not cause them to senesce. A significant reduction was observed in colony forming efficiency, anchorage independence and growth rate of cells transfected with RNaseT2. The levels of transcripts involved in Akt signalling pathway, cell cycle control and pathways related to cell proliferation decreased 2–10-folds in SV40 immortalized cells in response to RNaseT2 expression. Interestingly, some immortalized cells expressed alternatively spliced transcript variants instead of the full-length RNaseT2 transcript. Our results are consistent with the conclusion that RNaseT2 is a cell growth regulator and it does not induce senescence in SV40 immortalized cell lines.
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DOI：
10.1038/nrc1299
发表时间：
2004-03
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1073/pnas.92.20.9363
发表时间：
1995-09-26
影响因子：
11.1
作者：
DIMRI, GP;LEE, XH;CAMPISI, J
通讯作者：
CAMPISI, J
影响因子：
8
作者：
Liu, Y;Emilion, G;Ganesan, TS
通讯作者：
Ganesan, TS
影响因子：
8
作者：
Reddy, DE;Sandhu, AK;Kaur, GP
通讯作者：
Kaur, GP
DOI：
10.1073/pnas.91.12.5498
发表时间：
1994-06-07
影响因子：
11.1
作者：
SANDHU, AK;HUBBARD, K;ATHWAL, RS
通讯作者：
ATHWAL, RS