Accurate Proteomewide Measurement of Methionine Oxidation in Aging Mouse Brains.
Accurate Proteomewide Measurement of Methionine Oxidation in Aging Mouse Brains.
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DOI：
10.1021/acs.jproteome.2c00127
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发表时间：
2022-06-03
影响因子：
4.4
通讯作者：
Ghaemmaghami, Sina
中科院分区：
文献类型：
--
作者：
Bettinger, John Q.;Simon, Matthew;Korotkov, Anatoly;Welle, Kevin A.;Hryhorenko, Jennifer R.;Seluanov, Andrei;Gorbunova, Vera;Ghaemmaghami, Sina
蛋氨酸的氧化已成为蛋白质的重要翻译后修饰。许多研究表明，蛋氨酸在选择蛋白质的氧化可以对细胞生理学产生不同的影响，从对蛋白质稳定性的有害影响到细胞信号传导中的功能作用。尽管其重要性，蛋氨酸氧化在一个复杂的矩阵，如细胞蛋白质组的大规模调查，已受到技术限制。我们报告了一种方法，蛋氨酸氧化阻断（MobB），允许准确和精确的定量低水平的蛋氨酸氧化通常在体内观察到的。为了证明这种方法的实用性，我们分析了年轻人（6个月）的脑组织。和老（20 m.o.）小鼠，并确定了超过280个新的网站在体内蛋氨酸氧化。我们进一步证明了特定蛋氨酸残基的氧化化学计量在个体动物之间是高度一致的，并且蛋氨酸亚砜富集在功能相关的基因产物簇中，包括膜和细胞外蛋白。然而，我们没有检测到老年小鼠脑中蛋氨酸氧化的显着变化。我们的研究结果表明，在正常条件下，蛋氨酸氧化可能是一个生物调节的过程，而不是随机的化学损伤的结果。
The oxidation of methionine has emerged as an important post-translational modification of proteins. A number of studies have suggested that the oxidation of methionines in select proteins can have diverse impacts on cell physiology, ranging from detrimental effects on protein stability to functional roles in cell signaling. Despite its importance, the large-scale investigation of methionine oxidation in a complex matrix, such as the cellular proteome, has been hampered by technical limitations. We report a methodology, methionine oxidation by blocking (MobB), that allows for accurate and precise quantification of low levels of methionine oxidation typically observed in vivo. To demonstrate the utility of this methodology, we analyzed the brain tissues of young (6 m.o.) and old (20 m.o.) mice and identified over 280 novel sites for in vivo methionine oxidation. We further demonstrated that oxidation stoichiometries for specific methionine residues are highly consistent between individual animals and methionine sulfoxides are enriched in clusters of functionally related gene products including membrane and extracellular proteins. However, we did not detect significant changes in methionine oxidation in brains of old mice. Our results suggest that under normal conditions, methionine oxidation may be a biologically regulated process rather than a result of stochastic chemical damage.
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影响因子：
4.5
作者：
Bachmann-Gagescu R;Dona M;Hetterschijt L;Tonnaer E;Peters T;de Vrieze E;Mans DA;van Beersum SE;Phelps IG;Arts HH;Keunen JE;Ueffing M;Roepman R;Boldt K;Doherty D;Moens CB;Neuhauss SC;Kremer H;van Wijk E
通讯作者：
van Wijk E
影响因子：
2.9
作者：
Balog, Edward M.;Lockamy, Elizabeth L.;Thomas, David D.;Ferrington, Deborah A.
通讯作者：
Ferrington, Deborah A.
影响因子：
4.4
作者：
Bettinger, John Q.;Welle, Kevin A.;Ghaemmaghami, Sina
通讯作者：
Ghaemmaghami, Sina
DOI：
10.1016/0167-4838(94)90003-5
发表时间：
1994-02-16
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
影响因子：
--
作者：
FUJII, N;ISHIBASHI, Y;HARADA, K
通讯作者：
HARADA, K
影响因子：
8.8
作者：
Cannizzo ES;Clement CC;Morozova K;Valdor R;Kaushik S;Almeida LN;Follo C;Sahu R;Cuervo AM;Macian F;Santambrogio L
通讯作者：
Santambrogio L