Presence of Salmonella AvrA in colorectal tumor and its precursor lesions in mouse intestine and human specimens.
Presence of Salmonella AvrA in colorectal tumor and its precursor lesions in mouse intestine and human specimens.
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DOI：
10.18632/oncotarget.19052
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发表时间：
2017-08-15
期刊：
影响因子：
--
通讯作者：
Sun J
中科院分区：
文献类型：
--
作者：
Lu R;Bosland M;Xia Y;Zhang YG;Kato I;Sun J
直接支持人类受试者中沙门氏菌感染与结直肠癌之间关联的证据很少。众所周知，沙门氏菌感染会增加患胆囊癌的风险。AvrA是一种来自肠道沙门氏菌的细菌蛋白，在慢性感染中起着至关重要的作用。据我们所知，细菌AvrA的存在从未在人类样本中进行过研究。在这里，我们通过动物实验感染模型和人类临床标本，证明了AvrA在炎症性结直肠肿瘤及其前体病变中的存在和细胞定位。我们进行了一项新开发的AvrA血清学试验，以确定慢性感染小鼠血清样本中抗沙门氏菌AvrA抗体的存在。此外，我们在健康的人类粪便样本中检测了AvrA基因的存在，以推进人类人群中AvrA沙门氏菌的病原学研究。我们的研究表明这种细菌蛋白在人类结直肠癌中有潜在的作用。此外，我们的新血清学检测可能是识别结直肠癌风险增加个体的有用工具。
Evidence directly supporting an association between Salmonella infection and colorectal cancer in human subjects is sparse. It has been well recognized that Salmonella infection increases the risk of gallbladder cancer. AvrA, a bacterial protein from Salmonella enterica, plays a crucial role in establishing chronic infection. To our knowledge, the presence of the bacterial AvrA has never been studied in human samples. Here, we demonstrated the presence and cellular localization of AvrA in inflamed, colorectal tumor and its precursor lesions, using both animal experimental infection models and human clinical specimens. We performed a newly developed AvrA serological assay and to determine the presence of anti-Salmonella AvrA antibody in chronic infected mouse serum samples. Further, we tested the presence of AvrA gene in healthy human fecal samples, in order to advance etiological studies of Salmonella AvrA in human population. Our study suggests a potential role of this bacterial protein in human colorectal cancer. Moreover, our new serological assay may serve a useful tool to identify individuals at increased risk for colorectal cancer.
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影响因子：
4.8
作者：
Lin, Zhijie;Zhang, Yong-Guo;Sun, Jun
通讯作者：
Sun, Jun
影响因子：
29.4
作者：
Gradel, Kim O.;Nielsen, Hans L.;Nielsen, Henrik
通讯作者：
Nielsen, Henrik
影响因子：
4.1
作者：
Kerrinnes, Tobias;Zelas, Zohar Ben-Barak;Yaron, Sima
通讯作者：
Yaron, Sima
DOI：
10.1016/j.neo.2016.04.001
发表时间：
2016-05
期刊：
Neoplasia (New York, N.Y.)
影响因子：
--
作者：
Lu R;Wu S;Zhang YG;Xia Y;Zhou Z;Kato I;Dong H;Bissonnette M;Sun J
通讯作者：
Sun J
影响因子：
2.8
作者：
Huehn, Stephan;La Ragione, Roberto M.;Malorny, Burkhard
通讯作者：
Malorny, Burkhard