Influence of Primary Structure on Fragmentation of Native-Like Proteins by Ultraviolet Photodissociation.
Influence of Primary Structure on Fragmentation of Native-Like Proteins by Ultraviolet Photodissociation.
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DOI：
10.1021/jasms.1c00269
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发表时间：
2021-12-01
影响因子：
3.2
通讯作者：
Brodbelt JS
中科院分区：
文献类型：
--
作者：
Macias LA;Sipe SN;Santos IC;Bashyal A;Mehaffey MR;Brodbelt JS
利用天然质谱法和辅助技术分析气相中的天然样蛋白结构已成为结构生物学应用的有力工具。结合紫外光解离（UVPD），天然自顶向下的质谱法为蛋白质结构的骨干柔韧性、拓扑结构、氢键网络和构象变化提供了信息。虽然已知初级结构影响多肽和蛋白质在气相的解离，但其对UVPD促进的骨干断裂类型和位置的影响以及对天然样蛋白结构表征的伴随影响尚不清楚。在这里，通过跟踪10种片段类型（a, a+1, b, c, x, x+1, y, y - 1， y和z）的倾向来评估天然样蛋白的碎片化趋势，这些片段类型与包含bbb9600个片段离子的天然UVPD数据集中的初级结构有关。据报道，不同的碎片趋势产生了不同的碎片类型，这归因于激发态的直接解离途径和推测涉及内部转换后分子内振动能量重新分配的途径的结合。后一种途径被系统地评估，以证明质子迁移在通过UVPD产生“类似cid”片段中的作用，为天然样蛋白的表征提供了相关的见解。这里提出的碎片化趋势被设想为增强对蛋白质高阶结构的分析或在完整蛋白质的高通量分析中增强评分算法。
Analysis of native-like protein structures in the gas phase via native mass spectrometry and auxiliary techniques has become a powerful tool for structural biology applications. In combination with ultraviolet photodissociation (UVPD), native top-down mass spectrometry informs backbone flexibility, topology, hydrogen bonding networks, and conformational changes in protein structure. Although it is known that the primary structure affects dissociation of peptides and proteins in the gas phase, its effect on the types and locations of backbone cleavages promoted by UVPD and concomitant influence on structural characterization of native-like proteins is not well understood. Here, trends in the fragmentation of native-like proteins were evaluated by tracking the propensity of 10 fragment types (a, a+1, b, c, x, x+1, y, y−1, Y, and z) in relation to primary structure in a native-top down UVPD data set encompassing >9600 fragment ions. Differing fragmentation trends are reported for the production of distinct fragment types, attributed to a combination of both direct dissociation pathways from excited electronic states and those surmised to involve intramolecular vibrational energy redistribution after internal conversion. The latter pathways were systematically evaluated to evince the role of proton mobility in the generation of “CID-like” fragments through UVPD, providing pertinent insight into the characterization of native-like proteins. Fragmentation trends presented here are envisioned to enhance analysis of the protein higher-order structure or augment scoring algorithms in the high-throughput analysis of intact proteins.
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影响因子：
15
作者：
Bonner J;Lyon YA;Nellessen C;Julian RR
通讯作者：
Julian RR
影响因子：
7.4
作者：
Chen B;Brown KA;Lin Z;Ge Y
通讯作者：
Ge Y
DOI：
10.1016/j.bbrc.2014.02.041
发表时间：
2014-03-21
影响因子：
3.1
作者：
Catherman, Adam D.;Skinner, Owen S.;Kelleher, Neil L.
通讯作者：
Kelleher, Neil L.
影响因子：
5.7
作者：
Hall, Zoe;Hernandez, Helena;Marsh, Joseph A.;Teichmann, Sarah A.;Robinson, Carol V.
通讯作者：
Robinson, Carol V.
影响因子：
15
作者：
Cammarata, Michael B.;Thyer, Ross;Brodbelt, Jennifer S.
通讯作者：
Brodbelt, Jennifer S.