Identification of distinct cytokine/chemokine profiles in dermatomyositis with anti-transcriptional intermediary factor 1-γ antibody.
Identification of distinct cytokine/chemokine profiles in dermatomyositis with anti-transcriptional intermediary factor 1-γ antibody.
复制标题
使用抗转录中间因子 1-γ 抗体鉴定皮肌炎中不同的细胞因子/趋化因子谱。
DOI：
10.1093/rheumatology/keab625
复制
发表时间：
2021
期刊：
影响因子：
5.5
通讯作者：
Hua Cao
中科院分区：
文献类型：
--
作者：
Qiang Zhao;Yongheng Chen;L. Diao;Shimin Zhang;Dan Wu;F. Xue;Qunli Xia;Hao Li;Jie Zheng;Hua Cao
目标
抗转录中间因子1-γ（anti-TIF 1-γ）抗体（Ab）阳性表达的皮肌炎（DM）和临床无肌病性皮肌炎（CADM）患者具有不同的临床病理特征。我们的目的是确定细胞因子/趋化因子谱在抗TIF 1-γ阳性DM/CADM患者分类中的作用。
方法
采用Luminex 200全自动生化分析仪检测27例抗TIF 1-γ阳性DM/CADM患者血清中24种细胞因子/趋化因子水平。主成分分析（PCA）和无监督层次聚类用于减少变量和建立患者亚组。计算细胞因子/趋化因子水平与疾病活动性标志物之间的斯皮尔曼相关系数。
结果
在抗TIF 1-γ阳性DM/CADM患者中，确定了两个不同的患者群。CADM的诊断在聚类1中比聚类2中更常见（58.3% vs 6.7%，p = 0.008）。如通过CDASI-A测量的，簇2中的皮肤病活动性高于簇1（38.6 ± 10.4 vs 25.3 ± 10.0，p = 0.003）。第2组中的患者表现出显著肌无力（MRC ≤ 3，33.3% vs 0.0%，p = 0.047）、抗TIF 1-γ Ab水平较高（92.4 ± 20.6 vs 66.9 ± 13.9，p = 0.001）和恶性肿瘤发生率升高（73.3% vs 25.0%，p = 0.021）。聚类2显示出较高的血清CXCL 10水平，（564.2 ± 258.8 vs 122.0 ± 97.8，p < 0.001），CCL2（1136.6 ± 545.4 vs 441.6 ± 163.3，p < 0.001），半乳糖凝集素-9（38879.6 ± 20009.3 vs 12612.4 ± 6640.0，p < 0.001），IL-18（436.1 ± 188.9 vs 243.0 ± 114.5，p = 0.003）、TNF-α（9.3 ± 3.8 vs 5.6 ± 2.4，p = 0.007）和TNFRI（1385.1 ± 338.2 vs 2605.6 ± 928.5，p < 0.001）。
结论
在抗TIF 1-γ阳性DM/CADM中，我们根据细胞因子/趋化因子谱鉴定了“皮肤主导”簇和“过度炎症”簇。抗TIF 1-γ阳性DM/CADM中的细胞因子/趋化因子谱可以识别具有不同疾病模式、器官受累和临床结局的离散患者群。
OBJECTIVES
Dermatomyositis (DM) and clinically amyopathic dermatomyositis (CADM) patients with positive expression of anti-transcription intermediary factor 1-γ (anti-TIF1-γ) antibody (Ab) are characterized by distinct clinicopathological features. We aimed to determine the role of cytokine/chemokine profiles in the classification of anti-TIF1-γ positive DM/CADM patients.
METHODS
Serum levels of 24 cytokines/chemokines were measured in 27 anti-TIF1-γ positive DM/CADM patients by a Luminex 200 system. Principal components analysis (PCA) and unsupervised hierarchical clustering were used to reduce variables and establish patient subgroups. Spearman's correlation coefficient was calculated between cytokine/chemokine levels and disease activity markers.
RESULTS
Among anti-TIF1-γ positive DM/CADM patients, two distinct patient clusters were identified. The diagnosis of CADM was more common in Cluster 1 than in Cluster 2 (58.3% vs 6.7%, p = 0.008). Skin disease activity was higher in Cluster 2 than in Cluster 1 as measured by CDASI-A (38.6 ± 10.4 vs 25.3 ± 10.0, p = 0.003). Patients within Cluster 2 exhibited significant muscle weakness (MRC ≤ 3, 33.3% vs 0.0%, p = 0.047), higher levels of anti-TIF1-γ Ab (92.4 ± 20.6 vs 66.9 ± 13.9, p = 0.001), and an increased malignancy rate (73.3% vs 25.0%, p = 0.021). Cluster 2 exhibited higher serum levels of CXCL10 (564.2 ± 258.8 vs 122.0 ± 97.8, p < 0.001), CCL2 (1136.6 ± 545.4 vs 441.6 ± 163.3, p < 0.001), Galectin-9 (38879.6 ± 20009.3 vs 12612.4 ± 6640.0, p < 0.001), IL-18 (436.1 ± 188.9 vs 243.0 ± 114.5, p = 0.003), TNF-α (9.3 ± 3.8 vs 5.6 ± 2.4, p = 0.007), and TNFRI (1385.1 ± 338.2 vs 2605.6 ± 928.5, p < 0.001) than Cluster 1.
CONCLUSION
In anti-TIF1-γ positive DM/CADM, we identified a "skin-predominant" cluster and a "hyperinflammation" cluster based on the cytokine/chemokine profiles. Cytokine/chemokine profiles in anti-TIF1-γ positive DM/CADM can identify discrete clusters of patients with different disease patterns, organ involvements, and clinical outcomes.
影响因子：
13.8
作者：
Fiorentino, David F.;Kuo, Karen;Chung, Lorinda;Zaba, Lisa;Li, Shufeng;Casciola-Rosen, Livia
通讯作者：
Casciola-Rosen, Livia
影响因子：
--
作者：
Nagaraju, K;Casciola-Rosen, L;Raben, N
通讯作者：
Raben, N
影响因子：
--
作者：
Fiorentino, David F.;Chung, Lorinda S.;Christopher-Stine, Lisa;Zaba, Lisa;Li, Shufeng;Mammen, Andrew L.;Rosen, Antony;Casciola-Rosen, Livia
通讯作者：
Casciola-Rosen, Livia