Effects of weekly blood collection in C57BL/6 mice.
Effects of weekly blood collection in C57BL/6 mice.
复制标题
C57BL/6 小鼠每周采血的影响。
DOI：
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发表时间：
2011
影响因子：
1.7
通讯作者：
J. Fortman
中科院分区：
文献类型：
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作者：
Brigitte M Raabe;J. Artwohl;J. Purcell;J. Lovaglio;J. Fortman
我们在10至14周龄的C57 BL/6小鼠中连续采血后评估了血液学恢复、体重和行为。雄性和雌性小鼠（试验组为5至11只小鼠，全研究组为23至35只小鼠）每周一次采集其估计总血量（TBV）的15%、20%或25%，持续6周。除25%TBV雄性中试组外，所有小鼠的体重从一次收集到下一次收集均恢复或增加。除25%TBV雄性试验组外，所有小鼠的行为在整个研究期间均表现正常。在每次每周采血时分析红细胞计数值较基线的变化。恢复定义为平均血红蛋白值恢复至平均基线值的2 SD范围内。根据该定义，15%TBV雄性组和15%、20%和25%TBV雌性组中的小鼠在血液学上恢复。为了支持恢复的统计学定义，我们将我们的数据与人类贫血类别进行了比较，以评估小鼠血红蛋白值的临床相关性。基于这些数据，我们得出结论，每周一次可以从雌性小鼠中收集多达25%的TBV，持续6周，每周一次可以从雄性小鼠中收集多达15%的TBV，持续6周，而不会产生体重减轻、行为改变或临床上显着的贫血。
We assessed hematologic recovery, body weight, and behavior after serial blood collection in 10- to 14-wk-old C57BL/6 mice. Male and female mice (5 to 11 mice for pilot groups, 23 to 35 mice for full study groups) had either 15%, 20%, or 25% of their estimated total blood volume (TBV) collected once weekly for 6 wk. Except for those of the 25% TBV male pilot group, the weights of all mice recovered or increased from one collection to the next. The behavior of all mice, with the exception of the 25% TBV male pilot group, appeared normal throughout the study. Erythrogram value changes from baseline were analyzed at each weekly blood collection. Recovery was defined as the return of mean hemoglobin values to within 2 SD of mean baseline values. According to this definition, mice in the 15% TBV male group and 15%, 20%, and 25% TBV female groups recovered hematologically. To support the statistical definition of recovery, we compared our data with human anemia categories to assess the clinical relevance of the mouse hemoglobin values. On the basis of these data, we conclude that as much as 25% TBV can be collected once weekly from female mice for 6 wk and as much as 15% TBV can be collected once weekly from male mice for 6 wk without producing weight loss, behavioral changes, or clinically significant anemia.
影响因子：
120.7
作者：
Izaks, GJ;Westendorp, RGJ;Knook, DL
通讯作者：
Knook, DL