Gravity, a regulation factor in the differentiation of rat bone marrow mesenchymal stem cells.
Gravity, a regulation factor in the differentiation of rat bone marrow mesenchymal stem cells.
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重力是大鼠骨髓间充质干细胞分化的调节因素
DOI：
10.1186/1423-0127-16-87
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发表时间：
2009-09-21
影响因子：
11
通讯作者：
Li YH
中科院分区：
文献类型：
--
作者：
Huang Y;Dai ZQ;Ling SK;Zhang HY;Wan YM;Li YH
背景干细胞治疗已成为组织工程和再生医学的一种潜在治疗选择，但仍存在许多问题有待解决，如种子细胞的数量、定向分化和效率等。以前的一些研究集中在化学诱导微环境的研究。在本研究中，我们研究了重力对骨髓间充质干细胞（BMSCs）分化为力敏感细胞和力不敏感细胞的影响。方法和结果大鼠BMSCs（rBMSCs）在超重力和模拟微重力（SMG）条件下培养，有或无诱导培养基。采用免疫细胞化学、RT-PCR和Western-blot方法检测和分析细胞特征蛋白的表达水平。5-氮胞苷和超重力处理后，rBMSCs表达更多心肌细胞特征性蛋白，如cTnT、GATA 4和β-MHC，而SMG处理后这些蛋白表达较少。成骨诱导剂和超重力处理后，ColIA 1、Cbfa 1和ALP的表达水平明显升高。用SMG获得了相反的结果。用成脂诱导剂和SMG处理的rBMSCs表达更高水平的PPARgamma。如流式细胞仪分析所示，在无诱导剂的超重力条件下观察到更高水平的Cbfa 1或cTnT阳性细胞。这些结果表明，超重力诱导分化的rBMSCs的力敏感的细胞（心肌细胞和成骨细胞），而SMG诱导力不敏感的细胞（脂肪细胞）。ConclusionTogether，我们得出结论，重力是一个重要的因素影响的分化的rBMSCs，这提供了一个新的途径干细胞分化的机制研究和一种新的方法，以获得更多的承诺分化或未分化的细胞。
BackgroundStem cell therapy has emerged as a potential therapeutic option for tissue engineering and regenerative medicine, but many issues remain to be resolved, such as the amount of seed cells, committed differentiation and the efficiency. Several previous studies have focused on the study of chemical inducement microenvironments. In the present study, we investigated the effects of gravity on the differentiation of bone marrow mesenchymal stem cells (BMSCs) into force-sensitive or force-insensitive cells.Methods and resultsRat BMSCs (rBMSCs) were cultured under hypergravity or simulated microgravity (SMG) conditions with or without inducement medium. The expression levels of the characteristic proteins were measured and analyzed using immunocytochemical, RT-PCR and Western-blot analyses. After treatment with 5-azacytidine and hypergravity, rBMSCs expressed more characteristic proteins of cardiomyocytes such as cTnT, GATA4 and β-MHC; however, fewer such proteins were seen with SMG. After treating rBMSCs with osteogenic inducer and hypergravity, there were marked increases in the expression levels of ColIA1, Cbfa1 and ALP. Reverse results were obtained with SMG. rBMSCs treated with adipogenic inducer and SMG expressed greater levels of PPARgamma. Greater levels of Cbfa1- or cTnT-positive cells were observed under hypergravity without inducer, as shown by FACS analysis. These results indicate that hypergravity induces differentiation of rBMSCs into force-sensitive cells (cardiomyocytes and osteoblasts), whereas SMG induces force-insensitive cells (adipocytes).ConclusionTaken together, we conclude that gravity is an important factor affecting the differentiation of rBMSCs; this provides a new avenue for mechanistic studies of stem cell differentiation and a new approach to obtain more committed differentiated or undifferentiated cells.
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影响因子：
5.2
作者：
Kasper, Grit;Dankert, Niels;Duda, Georg N.
通讯作者：
Duda, Georg N.
影响因子：
2.7
作者：
Gebken, J;Lüders, B;Bätge, B
通讯作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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