Achieving accelerated osteogenic differentiation via novel magnesium silicate hollow spheres
Achieving accelerated osteogenic differentiation via novel magnesium silicate hollow spheres
复制标题
通过新型硅酸镁空心球实现加速成骨分化
DOI：
10.1039/c5nj02189h
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发表时间：
2015-11
影响因子：
3.3
通讯作者：
Wang Huiming
中科院分区：
文献类型：
--
作者：
Wang Baixiang;Wang Yu;Liu Chuanxia;Feng Xiaoxia;Yang Guoli;Wang Huiming
纳米材料因其多功能性而被广泛用作骨组织工程中的多功能支架和载体。本文引入经典的Stober方法，结合了无有机试剂水热路线的优点，合理设计并合成了新型硅酸镁空心球（记为MgSiO3空心球）。由于硅和镁等矿物元素的存在，这些精心准备的中空结构在加速成骨分化方面表现出积极的作用。以成骨细胞系MC3T3-E1为例，我们通过MTT测定、LDH测定、细胞凋亡和形态观察等多种经典方法研究了这些空心球的体外毒性。结果表明，这些材料具有极低的全身毒性和高生物相容性。此外，细胞内化观察表明，这些空心球可以以时间依赖性方式被MC3T3-E1细胞摄取。这种旺盛的内吞过程可以为后续的细胞内矿化和骨再生提供更多的原材料。 ALP 分析和茜素红染色结果表明，这些空心球可以以剂量依赖性方式明显促进成骨细胞分化和矿化。我们的研究表明，这些硅酸镁空心球可以作为增强骨再生和骨组织工程的有希望的候选者。
Nanomaterials have been widely used as multifunctional scaffolds and carriers in bone tissue engineering resulting from their versatile functionalities. Here, a classical Stober method that combined the merits of an organic reagent-free hydrothermal route was introduced to rationally design and synthesize novel magnesium silicate hollow spheres (denoted as MgSiO3 hollow spheres). Due to the presence of mineral elements including silicon and magnesium, these well-prepared hollow structures exhibited positive effects in accelerated osteogenic differentiation. By using an osteoblastic cell line as example, namely MC3T3-E1, we investigated the in vitro toxicity of these hollow spheres via various classical methods including MTT assay, LDH assay, apoptosis, and morphology observation. Results indicated that these materials revealed extremely low systemic toxicity and high bio-compatibility. Moreover, cellular internalization observation demonstrated that these hollow spheres could be taken up by MC3T3-E1 cells in a time-dependent manner. This vigorous process of endocytosis could provide more raw materials for the following intracellular mineralization and bone regeneration. Results of ALP analysis and alizarin red staining indicated that these hollow spheres could clearly promote osteoblast differentiation and mineralization in a dose-dependent manner. Our study suggested that these magnesium silicate hollow spheres could act as promising candidates for enhanced bone regeneration and bone tissue engineering.
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DOI：
10.1039/c5tb00843c
发表时间：
2015-08-21
期刊：
Journal of materials chemistry. B
影响因子：
--
作者：
Choi B;Cui ZK;Kim S;Fan J;Wu BM;Lee M
通讯作者：
Lee M
成骨细胞系 MC3T3-E1 中三种尺寸二氧化硅纳米粒子的特征
DOI：
10.1039/c4ra06863g
发表时间：
2014-09
期刊：
RSC Adv
影响因子：
--
作者：
D;an Wang;Wenhuan Bu;Changyu Zheng;Hongchen Sun
通讯作者：
Hongchen Sun
影响因子：
19
作者：
Fang, Qunling;Xuan, Shouhu;Gong, Xinglong
通讯作者：
Gong, Xinglong
影响因子：
6.7
作者：
Lee, Jong Ho;Shin, Yong Cheol;Han, Dong-Wook
通讯作者：
Han, Dong-Wook
DOI：
10.1016/j.colsurfb.2009.09.017
发表时间：
2010-02
期刊：
Colloids and surfaces. B, Biointerfaces
影响因子：
--
作者：
Z. Mao;L. Wan;Ling Hu;Lie Ma;Changyou Gao
通讯作者：
Z. Mao;L. Wan;Ling Hu;Lie Ma;Changyou Gao