The mother centriole plays an instructive role in defining cell geometry.
The mother centriole plays an instructive role in defining cell geometry.
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DOI：
10.1371/journal.pbio.0050149
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发表时间：
2007-06
期刊：
影响因子：
9.8
通讯作者：
Marshall WF
中科院分区：
文献类型：
--
作者：
Feldman JL;Geimer S;Marshall WF
中心粒定位是细胞几何构型建立和繁殖的关键步骤，但其定位机制尚不清楚。预先存在的中心粒能够以相对于它们自身的特定角度诱导形成新的中心粒，这表明它们可能有能力将空间信息传递给它们的子代。通过对衣藻细胞形态的三维计算机辅助分析，我们确定了中心粒相对于整个细胞极性定位所需的六个基因，其中四个基因具有已知的序列。我们发现，中心粒的远端部分对定位至关重要，中心粒定位细胞核，而不是相反。我们得到的证据表明，女儿的中心粒不能对正常的位置提示做出反应，而是依赖母亲提供位置信息。我们的结果代表了SonneBorn定义的“细胞趋向性”的一个明显的例子，并表明中心粒可以在细胞几何图形的一代又一代的传播中发挥关键作用。这里记录的中心粒正确定位所需的基因可能代表了一类新的睫状体疾病基因，其中的缺陷可能会导致纤毛位置紊乱和功能受损。细胞不仅仅是一袋袋同质的酶，而是有一个精确而复杂的内部结构。然而，定义这一架构的机制仍然不清楚。不同的细胞器如何在细胞内找到它们的适当位置？我们已经开始用遗传学的方法来解决一个特殊细胞器--中心粒的这个问题。我们的方法依赖于这样一个事实，即纤毛和鞭毛的组装需要中心粒，纤毛和鞭毛用于游泳。我们研究了单细胞绿藻衣藻，它利用鞭毛游向光源。我们筛选了不能游向光的突变体，发现了一组突变体，在这些突变体中，中心粒和鞭毛偏离了它们在细胞内的正常位置。利用这些突变体，我们获得了中心粒在定位细胞内其他结构(如细胞核)方面发挥作用的证据。我们还发现，在这些包含两个年龄不同的中心粒的细胞中，较老的中心粒在定位较新的中心粒方面发挥了作用，这表明细胞可能有一种方式将空间模式从一代传播到下一代。衣藻中发现了6个中心粒定位所需的基因。对这些基因突变的细胞的分析显示，中心粒在定位其他细胞器方面起着至关重要的作用。
Centriole positioning is a key step in establishment and propagation of cell geometry, but the mechanism of this positioning is unknown. The ability of pre-existing centrioles to induce formation of new centrioles at a defined angle relative to themselves suggests they may have the capacity to transmit spatial information to their daughters. Using three-dimensional computer-aided analysis of cell morphology in Chlamydomonas, we identify six genes required for centriole positioning relative to overall cell polarity, four of which have known sequences. We show that the distal portion of the centriole is critical for positioning, and that the centriole positions the nucleus rather than vice versa. We obtain evidence that the daughter centriole is unable to respond to normal positioning cues and relies on the mother for positional information. Our results represent a clear example of “cytotaxis” as defined by Sonneborn, and suggest that centrioles can play a key function in propagation of cellular geometry from one generation to the next. The genes documented here that are required for proper centriole positioning may represent a new class of ciliary disease genes, defects in which would be expected to cause disorganized ciliary position and impaired function. Cells are not just homogenous bags of enzymes, but instead have a precise and complex internal architecture. However, the mechanisms that define this architecture remain unclear. How do different organelles find their proper location within the cell? We have begun to address this question for one particular organelle, the centriole, using a genetic approach. Our approach relies on the fact that centrioles are required for the assembly of cilia and flagella, which are used for swimming. We studied the unicellular green alga Chlamydomonas, which use flagella to swim towards a light source. We screened for mutants that could not swim towards light, and found a set of mutants in which the centrioles and flagella are displaced from their normal location within the cell. Using these mutants, we have obtained evidence that centrioles play a role in positioning other structures within the cell, such as the nucleus. We also found that in these cells, which contain two centrioles differing in age, the older centriole plays a role in positioning the newer centriole, suggesting that cells may have a way to propagate spatial patterns from one generation to the next. Six genes required for centriole positioning are identified inChlamydomonas. Analysis of cells with mutations in these genes reveals a crucial role for the centriole in positioning other organelles.
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影响因子：
7.8
作者：
ADAMS, GMW;WRIGHT, RL;JARVIK, JW
通讯作者：
JARVIK, JW
DOI：
10.1083/jcb.102.5.1797
发表时间：
1986-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bloodgood RA;Woodward MP;Salomonsky NL
通讯作者：
Salomonsky NL
影响因子：
64.8
作者：
Cowan, CR;Hyman, AA
通讯作者：
Hyman, AA
影响因子：
64.5
作者：
HUANG, B;RAMANIS, Z;LUCK, DJL
通讯作者：
LUCK, DJL
影响因子：
3.2
作者：
HIRSCHBERG, R;STAVIS, R
通讯作者：
STAVIS, R