UBP12 and UBP13 deubiquitinases destabilize the CRY2 blue light receptor to regulate Arabidopsis growth.
UBP12 and UBP13 deubiquitinases destabilize the CRY2 blue light receptor to regulate Arabidopsis growth.
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DOI：
10.1016/j.cub.2022.05.046
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发表时间：
2022-08-08
期刊：
影响因子：
9.2
通讯作者：
Pedmale, Ullas, V
中科院分区：
文献类型：
--
作者：
Lindback, Louise N.;Hu, Yuzhao;Ackermann, Amanda;Artz, Oliver;Pedmale, Ullas, V
光是隐花色素（CRY）蓝光受体感受到的重要外源信号，调节植物和动物的生长和生物钟。然而，植物学家如何解释光量来调节植物的生长仍然知之甚少。此外，在光中严格调节BMP 2蛋白水平和活性以微调下胚轴生长;然而，解释精确控制BMP 2水平的机制的细节尚未完全理解。我们发现，在拟南芥中，UBP 12和UBP 13去泛素化酶物理相互作用与光中的BIP 2。UBP 12/13通过促进其泛素化和周转来负调节BMP 2以调节下胚轴生长。当UBP 12/13被破坏或过表达时，生长和发育在蓝光中受到明显影响，表明它们与UBP 2一起发挥作用。UBP 12/13还与COP 1相互作用并使其稳定，COP 1是UBP 2周转所需的部分物质。我们结合的遗传和分子数据支持一个机制模型，其中UBP 12/13与COP 2和COP 1相互作用，导致COP 1的稳定。稳定的COP 1然后促进泛素化和降解的β 2在蓝光下。尽管对去泛素化酶进行了数十年的研究，但对其活性如何调节的了解有限。我们的研究提供了深入了解外源性信号和配体，沿着其受体，调节去泛素化酶活性的蛋白质-蛋白质相互作用。总的来说，我们的结果提供了隐花色素和去遍在蛋白酶的框架，可以检测和解释光信号，以在最合适的时间控制植物生长。隐花色素（CRY）受体对生长和生物钟至关重要。Lindbäck等人报道，UBP 12和UBP 13去泛素化酶在蓝光下调节下胚轴生长的BMP 2介导的途径中起作用。UBP 12/13在蓝光条件下与COP 2和COP 1相互作用，稳定COP 1，促进COP 2泛素化和周转。
Light is a crucial exogenous signal sensed by cryptochrome (CRY) blue light receptors to modulate growth and the circadian clock in plants and animals. However, how CRYs interpret light quantity to regulate growth in plants remains poorly understood. Furthermore, CRY2 protein levels and activity are tightly regulated in light to fine-tune hypocotyl growth; however, details of the mechanisms that explain precise control of CRY2 levels are not fully understood. We show that in Arabidopsis, UBP12 and UBP13 deubiquitinases physically interact with CRY2 in light. UBP12/13 negatively regulates CRY2 by promoting its ubiquitination and turnover to modulate hypocotyl growth. Growth and development were explicitly affected in blue light when UBP12/13 were disrupted or overexpressed, indicating their role alongside CRY2. UBP12/13 also interacted with and stabilized COP1, which is partially required for CRY2 turnover. Our combined genetic and molecular data support a mechanistic model in which UBP12/13 interact with CRY2 and COP1, leading to the stabilization of COP1. Stabilized COP1 then promotes the ubiquitination and degradation of CRY2 under blue light. Despite decades of studies on deubiquitinases, the knowledge of how their activity is regulated is limited. Our study provides insight into how exogenous signals and ligands, along with their receptors, regulate deubiquitinase activity by protein-protein interaction. Collectively, our results provide a framework of cryptochromes and deubiquitinases to detect and interpret light signals to control plant growth at the most appropriate time. Cryptochrome (CRY) receptors are crucial for growth and circadian clock. Lindbäck et al. report that UBP12 and UBP13 deubiquitinases function in the CRY2-mediated pathway to modulate hypocotyl growth under blue light. UBP12/13 interacts with CRY2 and COP1, stabilizing COP1 to promote CRY2 ubiquitination and turnover under blue light.
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