Aligned Nanofibrous Cell-Derived Extracellular Matrix for Anisotropic Vascular Graft Construction.
Aligned Nanofibrous Cell-Derived Extracellular Matrix for Anisotropic Vascular Graft Construction.
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DOI：
10.1002/adhm.201601333
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发表时间：
2017-05
影响因子：
10
通讯作者：
Zhao F
中科院分区：
文献类型：
--
作者：
Xing Q;Qian Z;Tahtinen M;Yap AH;Yates K;Zhao F
血管重建手术或体外药物筛选组织模型的应用对组织工程血管移植物有大量需求。细胞外基质 (ECM) 成分以及天然血管的结构和机械各向异性对其功能性能至关重要。本研究的目的是通过采用纳米纤维排列的成纤维细胞衍生的 ECM 和人间充质干细胞 (hMSC) 来开发仿生血管移植物，重现天然血管的各向异性特征。对齐的 ECM 的纳米形貌线索指导了初始细胞方向。随后在旋转壁容器 (RWV) 生物反应器产生的圆周应力下的成熟进一步促进了移植物的各向异性结构和机械性能。生物反应器样品的周向拉伸强度显着高于纵向强度。在没有任何生化刺激的生物反应器样品中，hMSC 中平滑肌细胞特异性基因、α-平滑肌肌动蛋白和钙调蛋白的表达大大增强。此外，使用预制 ECM 和 RWV 生物反应器将移植物制造时间显着缩短至 3 周。我们研究中提出的血管移植物模仿 ECM 成分、细胞表型、结构和机械各向异性，有望用于血管重建手术或体外组织模型应用。
There is a large demand for tissue engineered vascular grafts for the application of vascular reconstruction surgery or in vitro drug screening tissue model. The extracellular matrix (ECM) composition, along with the structural and mechanical anisotropy of native blood vessels are critical to their functional performance. The objective of this study was to develop a biomimetic vascular graft recapitulating the anisotropic features of native blood vessels by employing nanofibrous aligned fibroblast-derived ECM and human mesenchymal stem cells (hMSCs). The nanotopographic cues of aligned ECM directed the initial cell orientation The subsequent maturation under circumferential stress generated by a rotating wall vessel (RWV) bioreactor further promoted anisotropic structural and mechanical properties in the graft. The circumferential tensile strength was significantly higher than longitudinal strength in bioreactor samples. Expression of smooth muscle cell specific genes, α-smooth muscle actin and calponin, in hMSCs was greatly enhanced in bioreactor samples without any biochemical stimulation. In addition, employment of pre-made ECM and RWV bioreactor significantly reduced the graft fabrication time to 3 weeks. Mimicking the ECM composition, cell phenotype, structural and mechanical anisotropy, the vascular graft presented in our study is promising for vascular reconstruction surgery or in vitro tissue model applications.
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影响因子：
3.5
作者：
Gavara N;Chadwick RS
通讯作者：
Chadwick RS
影响因子：
4.8
作者：
Gong, Zhaodi;Niklason, Laura E.
通讯作者：
Niklason, Laura E.
影响因子：
4.8
作者：
L'Heureux, N;Pâquet, S;Auger, FA
通讯作者：
Auger, FA
影响因子：
3.8
作者：
Arrigoni, Chiara;Chitto, Annalisa;Remuzzi, Andrea
通讯作者：
Remuzzi, Andrea
DOI：
10.1073/pnas.0704581104
发表时间：
2007-07-17
影响因子：
11.1
作者：
Hashi, Craig K.;Zhu, Yiqian;Li, Song
通讯作者：
Li, Song