Plasmon color-preserved gold nanoparticle clusters for high sensitivity detection of SARS-CoV-2 based on lateral flow immunoassay.
Plasmon color-preserved gold nanoparticle clusters for high sensitivity detection of SARS-CoV-2 based on lateral flow immunoassay.
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DOI：
10.1016/j.bios.2022.114094
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发表时间：
2022-06-01
影响因子：
12.6
通讯作者：
Kim MG
中科院分区：
文献类型：
--
作者：
Oh HK;Kim K;Park J;Im H;Maher S;Kim MG
侧流免疫分析 (LFI) 在即时检测 (POC) 传感应用方面显示出巨大的前景，但其临床转化往往因早期检测疾病（包括严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2)）的灵敏度不足而受到阻碍。这主要是由于单个金纳米颗粒（AuNP）的吸收信号较弱。在这里，我们开发了 AuNP 簇，它保持了孤立的单个 AuNP 的红色，但增加了比色读数以提高检测灵敏度。等离子保色 (PLASCOP) AuNP 簇只需将链霉亲和素包被的 AuNP 核心与包被生物素化抗体的卫星 AuNP 混合即可制成。生物素化抗体-链霉亲和素连接体形成超过 15 nm 的间隙，以避免 AuNP 之间的等离激元耦合，从而在保持等离激元颜色的同时增加整体光吸收。使用由 40 nm AuNP 组成的 PLASCOP AuNP 簇进行的 LFI 传感显示出对 SARS-CoV-2 核衣壳蛋白的高检测灵敏度，检测限 (LOD) 为 0.038 ng mL−1，分别比单个 15 nm 和 40 nm AuNP 缀合物低 23.8 倍和 5.9 倍。基于 PLASCOP AuNP 簇的 LFI 传感还对来自其他流感和冠状病毒的 SARS-CoV-2 核衣壳蛋白表现出良好的特异性。在临床可行性测试中，我们证明可以以 54 TCID50 mL−1 的 LOD 检测到人类唾液中掺入的 SARS-CoV-2 颗粒。开发的 PLASCOP AuNP 簇是有前景的比色传感报告基因，可提高 LFI 传感的灵敏度，适用于 SARS-CoV-2 检测之外的广泛 POC 传感应用。
Lateral flow immunoassays (LFI) have shown great promise for point-of-care (POC) sensing applications, however, its clinical translation is often hindered by insufficient sensitivity for early detection of diseases, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This is mainly due to weak absorption signals of single gold nanoparticles (AuNPs). Here, we developed AuNP clusters that maintain the red color of isolated individual AuNPs, but increase the colorimetric readout to improve the detection sensitivity. The plasmon color-preserved (PLASCOP) AuNP clusters is simply made by mixing streptavidin-coated AuNP core with satellite AuNPs coated with biotinylated antibodies. The biotinylated antibody-streptavidin linker forms a gap size over 15 nm to avoid plasmon coupling between AuNPs, thus maintaining the plasmonic color while increasing the overall light absorption. LFI sensing using PLASCOP AuNP clusters composed of 40 nm AuNPs showed a high detection sensitivity for SARS-CoV-2 nucleocapsid proteins with a limit of detection (LOD) of 0.038 ng mL−1, which was 23.8- and 5.9-times lower value than that of single 15 nm and 40 nm AuNP conjugates, respectively. The PLASCOP AuNP clusters-based LFI sensing also shows good specificity for SARS-CoV-2 nucleocapsid proteins from other influenza and coronaviruses. In a clinical feasibility test, we demonstrated that SARS-CoV-2 particles spiked in human saliva could be detected with an LOD of 54 TCID50 mL−1. The developed PLASCOP AuNP clusters are promising colorimetric sensing reporters that present improved sensitivity in LFI sensing for broad POC sensing applications beyond SARS-CoV-2 detection.
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影响因子：
6.4
作者：
Koczula KM;Gallotta A
通讯作者：
Gallotta A
影响因子：
168.9
作者：
Chan, Jasper Fuk-Woo;Yuan, Shuofeng;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
固相萃取后使用半胱胺功能化金纳米颗粒对大米样品中的莠去津进行比色传感
影响因子：
3.1
作者：
Liu, Guangyang;Wang, Shanshan;Shao, Hua
通讯作者：
Shao, Hua
影响因子：
3.5
作者：
Li, Yu;Chen, Xirui;Xiong, Yonghua
通讯作者：
Xiong, Yonghua
DOI：
10.1021/acs.jpcc.1c01071
发表时间：
2021-03-11
期刊：
The journal of physical chemistry. C, Nanomaterials and interfaces
影响因子：
--
作者：
Barr JW;Gomrok S;Chaffin E;Huang X;Wang Y
通讯作者：
Wang Y