Antioxidant and Antisenescence Effects of Bergamot Juice.
Antioxidant and Antisenescence Effects of Bergamot Juice.
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DOI：
10.1155/2018/9395804
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发表时间：
2018
影响因子：
--
通讯作者：
Martini C
中科院分区：
文献类型：
--
作者：
Da Pozzo E;De Leo M;Faraone I;Milella L;Cavallini C;Piragine E;Testai L;Calderone V;Pistelli L;Braca A;Martini C
衰老是心血管疾病发病的主要危险因素之一；其中一个可能的解释可能与年龄相关的自由基过度产生有关。这种氧化压力的增加可以通过摄入大量的食物抗氧化剂来克服。在这一背景下，一些研究已经被用来评估天然抗氧化剂化合物的抗衰老潜力。最近，有研究表明，佛手柑的果汁是一种主要产于意大利南部爱奥尼亚沿海地区(卡拉布里亚)的水果，其果汁是一种宝贵的健康促进成分来源，具有抗氧化等特性。为了研究这一地中海天然抗氧化剂来源的潜在抗衰老作用，采用高效液相色谱-光电二极管阵列-电喷雾电离-串联质谱仪对三个不同品种的佛手果汁(“Fantastico”、“Femminello”和“Castagnaro”)进行了表征。然后，在两种不同的诱导心肌衰老的细胞模型上，研究了果汁中总多酚和黄酮类的含量，无细胞模型的抗氧化活性，以及体外抗衰老性能。最好的果汁也在体内进行了评估。植物化学图谱证实，果汁中含有丰富的类黄酮类化合物，既有黄酮苷，也有黄酮苷。此外，在所有品种中还鉴定出两种柠檬苦苷。不同品种表现出不同的酚类和类黄酮含量。试管结果表明，果汁具有很强的抗氧化活性，这与其酚类和类黄酮含量有关。此外，在这两个细胞模型中，第一次证明了果汁对抗化学诱导的衰老的能力。最后，从小鼠心脏获得的体内数据证明，参与抗衰老和抗氧化反应的基因转录增加。总体而言，结果表明，佛手果汁具有抗氧化和抗衰老的作用，这使其适用于营养食品。
Aging is one of the main risk factor for the onset of cardiovascular diseases; one of the possible explanations could be linked to the age-associated overproduction of free radicals. This increase of oxidative stress can be overcome with a high intake of food antioxidants. In this context, a number of studies have been addressed to assess the antiaging potential of natural antioxidant compounds. Recently, it has been shown that the juice of bergamot (Citrus bergamia Risso et Poiteau), a fruit mostly produced in the Ionian coastal areas of Southern Italy (Calabria), is a valuable source of health-promoting constituents with, among other, antioxidant properties. In order to investigate the potential antiaging effects of this Mediterranean natural antioxidant source, bergamot juices of three different cultivars (“fantastico,” “femminello,” and “castagnaro”) were herein characterized by the mean of high-performance liquid chromatography-photodiode array-electrospray ionization-tandem mass spectrometry. Then, juices were investigated for the evaluation of total polyphenolic and flavonoid contents, cell-free model antioxidant activities, and in vitro antiaging properties on two different cellular models of induced myocardial senescence. The best performing juice was also assessed in vivo. The phytochemical profiles confirmed that juices were rich in flavonoids, both flavone and flavanone glycosides. In addition, two limonoid glycosides were also identified in all cultivars. Each cultivar showed different phenolic and flavonoid contents. In tube results showed the juice robust antioxidant activities that correlate with their phenolic and flavonoid contents. Moreover, for the first time, the ability of juice to counteract the chemical-induced senescence was here demonstrated in both cellular models. Lastly, the in vivo data obtained from mouse hearts evidenced an increase in transcription of genes involved in antiaging and antioxidant responses. The overall results suggest that bergamot juice exerts antioxidant and antisenescence effects, making it useful for nutraceutical purposes.
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影响因子：
6.1
作者：
Ferlazzo, Nadia;Cirmi, Santa;Navarra, Michele
通讯作者：
Navarra, Michele
影响因子：
2.6
作者：
Costa, Rosaria;Dugo, Paola;Mondello, Luigi
通讯作者：
Mondello, Luigi
影响因子：
2.3
作者：
Ledesma-Escobar, C. A.;Priego-Capote, F.;Luque de Castro, M. D.
通讯作者：
Luque de Castro, M. D.
影响因子：
--
作者：
Armentano, Maria Francesca;Bisaccia, Faustino;Milella, Luigi
通讯作者：
Milella, Luigi
影响因子：
6.3
作者：
Impellizzeri, Daniela;Bruschetta, Giuseppe;Navarra, Michele
通讯作者：
Navarra, Michele