The plant hormone ethylene restricts Arabidopsis growth via the epidermis.
The plant hormone ethylene restricts Arabidopsis growth via the epidermis.
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DOI：
10.1073/pnas.1717649115
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发表时间：
2018-04-24
影响因子：
11.1
通讯作者：
Van Der Straeten D
中科院分区：
文献类型：
--
作者：
Vaseva II;Qudeimat E;Potuschak T;Du Y;Genschik P;Vandenbussche F;Van Der Straeten D
乙烯是一种气态激素，在整个发育过程中控制植物生命。为简单的疏水性分子，可自由进入细胞；因此，其作用的细胞类型特异性具有挑战性。通过信号级联的两个负调节因子的组织特异性表达，我们选择性地破坏不同细胞类型中的乙烯信号而不影响其生物合成。我们证明乙烯通过抑制最外层细胞层中生长素的作用来限制植物生长。我们进一步表明，这种表皮特异性信号传导对邻近细胞的生长有影响，这表明细胞扩张的主控制器位于表皮，它感知环境并随后驱动内部组织的生长。气态激素乙烯在植物生长和发育中起着关键作用，并且是应激反应的主要调节剂。它主要通过与生长素的相互作用来限制细胞伸长，从而抑制营养生长。然而，目前尚不清楚乙烯是否控制所有植物组织的生长或其信号传导是否仅限于特定细胞类型。我们采用靶向表达方法来绘制乙烯生长调节的组织位点。泛素 E3 连接酶复合物含有 Skp1、Cullin1 和 F-box 蛋白 EBF1 或 EBF2 (SCFEBF1/2)，其目标是降解 EIN3（乙烯信号转导中的主要转录因子）。我们将 EBF1 和 EBF2 与许多细胞类型特异性启动子偶联。通过乙烯反应和突变体互补的表型测定，我们发现表皮是乙烯作用控制植物根和芽生长的主要部位。抑制组成型乙烯信号传导突变体 ctr1-1 表皮中的乙烯信号传导足以挽救突变表型，表明表皮是乙烯介导的生长抑制所需的关键细胞类型。
Ethylene is a gaseous hormone that controls plant life throughout development. Being a simple hydrophobic molecule, it can freely enter cells; therefore, the cell type specificity of its action is challenging. By means of tissue-specific expression of two negative regulators of the signaling cascade, we selectively disrupted the ethylene signal in different cell types without affecting its biosynthesis. We demonstrate that ethylene restricts plant growth by dampening the effect of auxins in the outermost cell layer. We further show that this epidermis-specific signaling has an impact on the growth of neighboring cells, suggesting that the master controller of cell expansion resides in the epidermis, where it senses the environment and, subsequently drives growth, of the inner tissues. The gaseous hormone ethylene plays a key role in plant growth and development, and it is a major regulator of stress responses. It inhibits vegetative growth by restricting cell elongation, mainly through cross-talk with auxins. However, it remains unknown whether ethylene controls growth throughout all plant tissues or whether its signaling is confined to specific cell types. We employed a targeted expression approach to map the tissue site(s) of ethylene growth regulation. The ubiquitin E3 ligase complex containing Skp1, Cullin1, and the F-box protein EBF1 or EBF2 (SCFEBF1/2) target the degradation of EIN3, the master transcription factor in ethylene signaling. We coupled EBF1 and EBF2 to a number of cell type-specific promoters. Using phenotypic assays for ethylene response and mutant complementation, we revealed that the epidermis is the main site of ethylene action controlling plant growth in both roots and shoots. Suppression of ethylene signaling in the epidermis of the constitutive ethylene signaling mutant ctr1-1 was sufficient to rescue the mutant phenotype, pointing to the epidermis as a key cell type required for ethylene-mediated growth inhibition.
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影响因子：
64.8
作者：
Fu, XD;Harberd, NP
通讯作者：
Harberd, NP
DOI：
10.1073/pnas.1613499114
发表时间：
2017-06-13
影响因子：
11.1
作者：
Barbez, Elke;Duenser, Kai;Busch, Wolfgang
通讯作者：
Busch, Wolfgang
影响因子：
5.6
作者：
Barrada A;Montané MH;Robaglia C;Menand B
通讯作者：
Menand B
影响因子：
6.9
作者：
Dugardeyn, Jasper;Vandenbussche, Filip;Van Der Straeten, Dominique
通讯作者：
Van Der Straeten, Dominique
影响因子：
7.4
作者：
CHADWICK, AV;BURG, SP
通讯作者：
BURG, SP